Head-worn displays (HWDs) as timely condition monitoring are increasingly used in aviation. However, interface design characteristics that mainly affect HWD use have not been fully investigated. The aim of this study was to examine the effects of several important interface design characteristics (i.e., the distance between calibration lines and the layouts of vertical and horizontal scale belts) on task performance and user preference between different conditions of display, i.e., HWD or head-up display (HUD). Thirty participants joined an experiment in which they performed flight tasks. In the experiment, the calibration lines’ distance was set to three different levels (7, 9 and 11 mrad), and the scale belt layouts included horizontal and vertical scale belt layouts. The scale belts were set as follows: the original vertical scale belt width was set as L, and the horizontal scale belt height as H. The three layouts of the vertical calibration scale belt used were 3/4H, H and 3H/2. Three layouts of horizontal calibration scale belts were selected as 3L/4, L and 3L/2. The results indicated that participants did better with the HWD compared to the HUD. Both layouts of vertical and horizontal scale belts yielded significant effects on the users’ task performance and preference. Users showed the best task performance while the vertical calibration scale belts were set as H and horizontal calibration scale belts were set as L, and users generally preferred interface design characteristics that could yield an optimal performance. These findings could facilitate the optimal design of usable head-worn-display technology.