Phenotypic plasticity is considered a major mechanism that allows plants to adapt to heterogeneous environments. The physiological integration between the interconnected rhizomes or stolons of clonal plants influences the plasticity of such plants in heterogeneous environments. However, the determinants of plasticity of reproductive ramets in clonal plants in homogeneous environments are unclear. Here, we chose Leymus chinensis, a perennial rhizomatous grass, and conducted a series of field experiments in situ, including grading sampling of reproductive ramets and different connection forms of vegetative ramets labeled with 15 N at four reproductive stages. Reproductive ramet biomass, inflorescence biomass, seed number, seed-setting percentage, reproductive allocation, and reallocation significantly increased with an increase in the number of vegetative ramets connected to tillering nodes, and the plasticity indexes of these six phenotypic characteristics showed similar increasing trends. The amount of nutrients supplied from the connected vegetative ramets to the reproductive ramets was significantly affected by the transfer direction, reproductive stage, and position order of the vegetative ramets. Throughout the sexual reproduction stage, nutrients were preferentially transferred to the acropetal reproductive ramet in L. chinensis populations. The amount of nutrients supplied from the connected vegetative ramets to the reproductive ramets at the milk-ripe stage, when sexual reproduction was most vigorous, was significantly larger than that at other reproductive stages. The amount of nutrients supplied from the spacer vegetative ramet to the acropetal reproductive ramet was significantly larger than that to the basipetal reproductive ramet. The closer the vegetative ramet was to the reproductive ramet, the more nutrients were supplied; the amount of nutrients supplied was significantly negatively related to the position order of the vegetative ramet. We identified the determinant of plasticity in sexual reproduction in clonal plants in a homogeneous environment: physiological integration between ramets within clones. Our results are vital for better understanding the adaptation of populations and even the evolution of species of clonal plants.

Clonal plants usually reproduce asexually through vegetative propagation and sexually by producing seeds. Physiological integration, the translocation of essential resources between ramets, usually improves vegetative reproduction. However, how physiological integration affects sexual reproduction has been less studied in clonal grasses. Here, we chose Hierochloe glabra, a major early spring forage of the eastern Eurasian steppe, and conducted a series of field experiments, including sampling reproductive ramets connected by tillering nodes to different numbers of vegetative ramets and 15N leaf labeling of ramet pairs at the seed-filling stage. In the natural populations of H. glabra, vegetative ramets were taller, had more and larger leaves, and greater biomass than reproductive ramets. Except for reproductive ramet biomass, sexual reproductive characteristics significantly increased with an increase in the number and biomass of vegetative ramets connected to tillering nodes. 15N labeling showed that vegetative ramets supplied nutrients to reproductive ramets through tillering nodes. Overall, our results indicate that significant differences in morphological characteristics and biomass allocation underlie resources translocation from vegetative ramets towards reproductive ramets. Physiological integration between different functional ramets can increase sexual reproductive performance, which will be beneficial to population persistence in H. glabra.

Air-stable and nontoxic inorganic single-source precursor sodium yttrium fluorocarbonate was decomposed to complex fluoride and oxyfluoride nanocrystals under mild hydrothermal conditions and thermal treatments. Different reaction parameters such as solvent, reaction time, and temperature were studied for the phase, size, and morphology control of the precursor and product nanocrystallines. The size and morphology of the product nanocrystallines are determined by the precursor. Eu3+-doped NaYF4 and YOF nanocrystallines were used for the photoluminescence examinations.

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