Members of the genus Marinomonas are known for their environmental adaptation and metabolically versatility, with abundant proteins associated with antifreeze, osmotic pressure resistance, carbohydrase and multiple secondary metabolites. Comparative genomic analysis focusing on secondary metabolites and orthologue proteins were conducted with 30 reference genome sequences in genus Marinomonas. In this study, a Gram-stain-negative, rod-shaped, non-agellated and strictly aerobic bacterium, designated as strain E8 T , was isolated from the red algae (Gelidium amansii) in the coastal of Weihai, China. Optimal growth of the strain E8 T was observed at temperatures 25-30 ℃, pH 6.5-8.0 and 1-3% (w/v) NaCl. The DNA G + C content was 42.8 mol%. The predominant quinone was Q-8 and the major fatty acids were C 16:0 , summed feature 3 and summed feature 8. The main polar lipids were phosphatidylglycerol (PG) and phosphatidylethanolamine (PE). Based on data obtained from this polyphasic taxonomic study, strain E8 T belongs to a novel species of the genus Marinomonas, for which the name Marinomonas algarum is proposed. The type strain is E8 T (= KCTC 92201 T = MCCC 1K07070 T ).

Members of the genus Marinomonas are known for their environmental adaptation and metabolically versatility, with abundant proteins associated with antifreeze, osmotic pressure resistance, carbohydrase and multiple secondary metabolites. Comparative genomic analysis focusing on secondary metabolites and orthologue proteins were conducted with 30 reference genome sequences in genus Marinomonas. In this study, a Gram-stain-negative, rod-shaped, non-flagellated and strictly aerobic bacterium, designated as strain E8T, was isolated from the red algae (Gelidium amansii) in the coastal of Weihai, China. Optimal growth of the strain E8T was observed at temperatures 25–30 ℃, pH 6.5-8.0 and 1–3% (w/v) NaCl. The DNA G + C content was 42.8 mol%. The predominant quinone was Q-8 and the major fatty acids were C16:0, summed feature 3 and summed feature 8. The main polar lipids were phosphatidylglycerol (PG) and phosphatidylethanolamine (PE). Based on data obtained from this polyphasic taxonomic study, strain E8T belongs to a novel species of the genus Marinomonas, for which the name Marinomonas algarum is proposed. The type strain is E8T (= KCTC 92201T = MCCC 1K07070T).

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A Gram-stain-negative, strictly aerobic, non-flagellated, oxidase- and catalase-positive, rod-shaped marine bacterium, designated strain DM8T, was isolated from the intestine of Trichiurus japonicus in Weihai, China. The strain optimally grew at 25-35℃, with 1.0-4.0% (w/v) NaCl and at pH 7.0-8.0. Its colonies were circular, slightly yellow, non-transparent, smooth, and approximately 0.8-1.5 mm in diameter, after being cultured for 48h on marine agar 2216. Based on the result of phylogenetic analysis of 16S rRNA gene sequence, strain DM8T had close relationship with Oceanisphaera profunda SM1222T (96.9%) and the similarity between DM8T and the type strain Oceanisphaera litoralis DSM 15406T was 94.7%. Genome sequencing revealed a genome size of 3,109,059 bp and a G+C content of 46.9 mol%. It had Q-8 as the sole respiratory quinone and possessed C16:0, summed features 3 (C16:1ω7c/C16:1ω6c) and summed features 8 (C18:1ω7c/C18:1ω6c) as major fatty acids. The major polar lipid profile was composed of phosphatidylglycerol and phosphatidylethanolamine. Based on the phenotypic, chemotaxonomic characterisations, phylogenetic properties and genome analysis, strain DM8T should represent a novel species of the genus Oceanisphaera, for which the name Oceanisphaera pacifica sp. nov. is proposed. The type strain is DM8T (=KCTC 82764T =MCCC 1K06133T).