No abstract

Aquaporin 4 (AQP4) is a recently discovered membrane bound water-selective channel and has been described at the light microscopic level to be predominantly expressed in the astrocytes of the brain, especially at the perivascular astrocyte endfoot processes. Alpha1-syntrophin, a member of dystrophin-associated protein, has also been reported at the light microscopic to be expressed level in the same site of astrocytes as AQP4 and interacts with other molecules through its PDZ domain. AQP4 expression has been reported to be absent at the sarcolemma and the perivascular astrocyte endfoot processes of alpha1-syntrophin knockout mice. Based on these observations, the molecular association between AQP4 and alpha1-syntrophin could be speculated. To test this hypothesis, we investigated the ultrasturctural localization of AQP4 and alpha1-syntrophin in the brain astrocytes by using double immunogold labeled electron microscopy. The results showed that AQP4 and alpha1-syntrophin colocalized frequently at the astrocyte membrane, especially at the perivascular astrocyte endfoot processes and suggested the presence of linkage between AQP4 and alpha1-syntrophin at the astrocyte plasma membrane.

This paper states evaluative methods of technology readiness which are used in the national background projects development. The methods conduct helicopter transmission system technology maturity research and assessment of native significant project according to methods of technology readiness assessment. The conclusions indicate that methods of technology readiness assessment have very important promoting action to native project development and scientific decision-making. The project which adopts mature critical technology implies that guarantee the schedule of project development and reduce risk of project development. Actualizing assess methods of technology readiness can be an important part of project development risk management.

In the pipeline construction, the girth welds tend to be the weakness because of defects and microstructural heterogeneities. The importance of suitable assessment of various defects in the weld is not only to prevent the cracks from unstable growth to cause catastrophic accident but also can effectively reduce the weld repair to reduce construction cost. Although many welding defects assessment methods and codes have been applied in this field, there are many differences among them. In this paper, the application of weld defect assessment methods was extensively studied. The key points of ECA applications, such as the pipeline axial stress and toughness, have been introduced. Furthermore, some suggestions were given on the application of girth weld ECA assessment.