Peroxynitrite (ONOO−) is a potent bio-oxidant involved in many physiological and pathological processes, however, most of the pathological effects associated with ONOO−in vivo are still ambiguous. Herein, we design and...
Phagocyte respiratory burst in immune response generate enormous amounts of ROS to fulfill primary defense against neoplasia. However, the beneficial functions associated with ROS especially the potent oxidant/nucleophile peroxynitrite, in immunological process is still ambiguous. Herein we report the construction and biological assessment of cyanine-based fluorescent biosensors which were based on non-oxidative strategy for peroxynitrite detection. The established nonoxidative strategy is comprised of nucleophilic substitution and nano-aggregates formation initiated by peroxynitrite. The proposed non-oxidative strategy in this study could maintain cellular oxidative stress in the critical process of detection and preserve homeostasis of cell metabolism. The remarkable detection sensitivity, reaction selectivity, and spectral photo-stability of our biosensors enabled us to visualize endogenous peroxynitrite levels in immune stimulated phagocytes. With the aid of basal peroxynitrite imaging in an acute peritonitis model, the visualization of peroxynitrite level variations in immune response of tumorigenesis was accomplished assisted by our biosensors. It is envisioned that our strategy provided a promising tool for early tumor diagnosis and evaluation of tumor suppression in the process of immune responses without disturbing the functions of ROS signaling transduction.
The medium Ks value of copper complex contributed to the specific reduction of Cu2+ by homocysteine and the formation of a stable six-membered ring species.
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