Background Gastric cancer (GC) is a leading cause of cancer deaths, and an increased number of GC patients adopt to next-generation sequencing (NGS) to identify tumor genomic alterations for precision medicine. Methods In this study, we established a hybridization capture-based NGS panel including 612 cancer-associated genes, and collected sequencing data of tumors and matched bloods from 153 gastric cancer patients. We performed comprehensive analysis of these sequencing and clinical data. Results 35 significantly mutated genes were identified such as TP53 , AKAP9 , DRD2 , PTEN , CDH1 , LRP2 et al. Among them, 29 genes were novel significantly mutated genes compared with TCGA study. TP53 is the top frequently mutated gene, and tends to mutate in male (p = 0.025) patients and patients whose tumor located in cardia (p = 0.011). High tumor mutation burden (TMB) gathered in TP53 wild-type tumors (p = 0.045). TMB was also significantly associated with DNA damage repair (DDR) genes genotype (p = 0.047), Lauren classification (p = 1.5e−5), differentiation (1.9e−7), and HER2 status (p = 0.023). 38.31% of gastric cancer patients harbored at least one actionable alteration according to OncoKB database. Conclusions We drew a comprehensive mutational landscape of 153 gastric tumors and demonstrated utility of target next-generation sequencing to guide clinical management. Electronic supplementary material The online version of this article (10.1186/s12967-019-1941-0) contains supplementary material, which is available to authorized users.

Summary Sialic acid (SA) is an important nutrient but few studies have examined the link between dietary intake and breast milk sialic acid. The purpose of this observational study was to assess the potential relationship between human breast milk sialic acid levels and dietary nutrition intake 40 d postpartum. The study population included 90 healthy women who were lactating. Human breast milk SA concentrations were measured using fluorescence detector-high performance liquid chromatography (HPLC-FLD) analysis and nutritional intake was estimated by a computerized validated food frequency questionnaire. SA in human breast milk was bound to free oligosaccharides (82.35%), protein (15.27%) and free sialic acid (2.37%). The findings of this study indicate that subjects with higher milk SA levels showed statistically higher levels of vitamin A compared with subjects with lower SA levels (423.486172.29 vs 602.226126.46 mg/d, p50.000). In addition, there was a certain association (standardized coefficients50.713; p50.000) between breast milk SA and vitamin A intake in healthy young subjects. This study demonstrated that dietary vitamin A intake has a certain relationship with breast milk SA concentrations. This may be attributed to the influence of vitamin A on sialic acid glycoprotein and sialic acid mucopolysaccharide in the human body or the common food sources for vitamin A and sialic acid. Additional study is required to further investigate this relationship.

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