Pentoxifylline exhibits complex functions with extensive pharmacological effects and is used therapeutically due to its therapeutic effects and rapid metabolism in the body, with no cumulative effects and few side effects. The present study investigated the effects of pentoxifylline on cerebral ischemia reperfusion‑induced injury (IRI) through suppression of inflammation in rats. Hematoxylin and eosin staining was performed to evaluate the number of neurocytes, and ELISAs were applied to measure tumor necrosis factor‑α, interleukin‑6, malondialdehyde and superoxide dismutase activities. Treatment with pentoxifylline significantly recovered the cerebral ischemia reperfusion‑induced neurological deficit score and cerebral infarct volume in rats. In addition, pentoxifylline treatment significantly reversed the cerebral ischemia reperfusion‑induced interleukin‑6, tumor necrosis factor‑α, malondialdehyde and superoxide dismutase levels in vivo. Furthermore, pentoxifylline significantly inhibited cyclooxygenase‑2 and inducible nitric oxide synthase mRNA and protein expression in cerebral IRI mice. Treatment with pentoxifylline also significantly suppressed the expression of cleaved caspase‑3 and p38 mitogen‑activated protein kinase (MAPK) protein in cerebral IRI mice. These results indicate that the protective effects of pentoxifylline on cerebral IRI may occur via the p38 MAPK signaling pathway.

Supercritical carbon dioxide (SC-CO 2 ) extraction of whole fruit oil from Gardenia jasminoides Ellis was performed. The effect of extraction pressure, temperature and CO 2 flow rate on the oil yield was investigated by response surface methodology (RSM). The results showed that experimental data had a good fit to the proposed model (R 2 = 0.938). Extraction pressure, CO 2 flow rate, the quadratics of pressure, and the interaction between pressure and flow rate showed significant effects on the oil yield (p \ 0.05). The optimum parameters that maximized the yield of gardenia fruit oil (GFO) were: extraction pressure of 36.8 MPa, temperature of 65°C, and CO 2 flow rate of 15 kg/h. The main fatty acid of GFO was linoleic acid (about 44%), followed by palmitic acid (about 26.4%) and oleic acid (about 24.6%). a-Tocopherol was dominant in the total tocopherols of GFO, and showed the main antioxidant activity. The fatty acid composition and tocopherols content of GFO were not remarkably affected by the extraction by SC-CO 2 and n-hexane.

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