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a b s t r a c tThe aims of this study were: (1) the study of cadmium (Cd) accumulation and toxicity in different castor cultivars (Ricinus communis L.); (2) to investigate changes in antioxidant enzymatic activities and the subcellular distribution of Cd in young and old leaves from two different castor cultivars, after exposure to two different Cd concentrations, and explore the underlying mechanism of Cd detoxification focusing on antioxidant enzymes and subcellular compartmentalization. The Cd concentration, toxicity, and subcellular distribution, as well as superoxide dismutase (SOD), catalase (CAT), and peroxidase (POD) activities were measured in Zibo-3 and Zibo-9 cultivars after exposure to two different concentrations of Cd (2 mg/L and 5 mg/L) for 10 days. This research revealed Cd accumulation characteristics in castor are root4 stem4 young leaf 4old leaf. Castor tolerance was Cd dose exposure and the cultivars themselves dependent. Investigation of subcellular Cd partitioning showed that Cd accumulated mainly in the heat stable protein (HSP) and cellular debris fractions, followed by the Cd rich granule (MRG), heat denatured protein (HDP), and organelle fractions. With increasing Cd concentration in nutrient solution, the decreased detoxified fractions (BDM) and the increased Cd-sensitive fractions (MSF) in young leaves may indicate the increased Cd toxicity in castor cultivars. The BDM-Cd fractions or MSF-Cd in old leaves may be linked with Cd tolerance of different cultivars of castor. The antioxidant enzymes that govern Cd detoxification were not found to be active in leaves. Taken together, these results indicate Cd tolerance and toxicity in castor can be explained by subcellular partitioning.Crown

The rainfed cropland belt in Australia is of great importance to the world grain market but has the highest climate variability of all such regions globally. However, the spatial-temporal impacts of climate variability on crops during different crop growth stages across broadacre farming systems are largely unknown. This study aims to quantify the contributions of climate and Land Surface Temperature (LST) variations to the variability of the Enhanced Vegetation Index (EVI) by using remote sensing methods. The datasets were analyzed at an 8-day timescale across the rainfed cropland of eastern Australia. First, we found that EVI values were more variable during the crop reproductive growth stages than at any other crop life stage within a calendar year, but nevertheless had the highest correlation with crop grain yield (t ha-1). Second, climate factors and LST during the crop reproductive growth stages showed the largest variability and followed a typical east-west gradient of rainfall and a north-south temperature gradient across the study area during the crop growing season. Last, we identified two critical 8-day periods, beginning on day of the year (DoY) 257 and 289, as the key 'windows' of crop growth variation that arose from the variability in climate and LST. Our results show that the sum of the variability of the climate components within these two 8-day 'windows' explained >88% of the variability in the EVI, with LST being the dominant factor. This study offers a fresh understanding of the spatial-temporal climate-crop relationships in rainfed cropland and can serve as an early warning system for agricultural adaptation in broadacre rainfed cropping practices in Australia and worldwide.

Long-term maps of within-field crop yield can help farmers understand how yield varies in time and space and optimise crop management. This study investigates the use of Landsat NDVI sequences for estimating wheat yields in fields in Western Australia (WA). By fitting statistical crop growth curves, identifying the timing and intensity of phenological events, the best single integrated NDVI metric in any year was used to estimate yield. The hypotheses were that: (1) yield estimation could be improved by incorporating additional information about sowing date or break of season in statistical curve fitting for phenology detection; (2) the integrated NDVI metrics derived from phenology detection can estimate yield with greater accuracy than the observed NDVI values at one or two time points only. We tested the hypotheses using one field (~235 ha) in the WA grain belt for training and another field (~143 ha) for testing. Integrated NDVI metrics were obtained using: (1) traditional curve fitting (SPD); (2) curve fitting that incorporates sowing date information (+SD); and (3) curve fitting that incorporates rainfall-based break of season information (+BOS). Yield estimation accuracy using integrated NDVI metrics was further compared to the results using a scalable crop yield mapper (SCYM) model. We found that: (1) relationships between integrated NDVI metrics using the three curve fitting models and yield varied from year to year; (2) overall, +SD marginally improved yield estimation (r = 0.81, RMSE = 0.56 tonnes/ha compared to r = 0.80, RMSE = 0.61 tonnes/ha using SPD), but +BOS did not show obvious improvement (r = 0.80, RMSE = 0.60 tonnes/ha); (3) use of integrated NDVI metrics was more accurate than SCYM (r = 0.70, RMSE = 0.62 tonnes/ha) on average and had higher spatial and yearly consistency with actual yield than using SCYM model. We conclude that sequences of Landsat NDVI have the potential for estimation of wheat yield variation in fields in WA but they need to be combined with additional sources of data to distinguish different relationships between integrated NDVI metrics and yield in different years and locations.

Seasonal climate is the main driver of crop growth and yield in broadacre grain cropping systems. With a 40-year record of 30 m resolution images and 16-day revisits, the Landsat satellite series is ideal for producing long-term records of remotely sensed phenology to build understanding of how climate affects crop growth. However, the time-series of Landsat images exhibits gaps caused by cloud cover, which is common in wet periods when crops reach maximum growth. We propose a novel spatial–temporal approach to gap-filling that avoids data fusion. Crop growth curve estimation is used to perform temporal smoothing and incorporation of spatial weights allows spatial smoothing. We tested our approach using Landsat NDVI data acquired for an 8000 ha study area in Western Australia using a train/test approach where 157 available Landsat-7 images between 2013 and 2019 were used to train the model, and 95 at least 80% cloud-free Landsat-8 images from the same period were used to test its performance. We found that compared to nonspatial estimation, use of spatial weights in growth curve estimation improved correlation between observed and predicted NDVI by 75%, MAE by 31% and RMSE by 75%. For cropland, the correlation is improved by 58%, the MAE by 36% and the RMSE by 76%. We conclude that spatially weighted estimation of crop growth curves can be used to fill spatial and temporal gaps in Landsat NDVI for the purpose of within-field monitoring. Our approach is also applicable to other data sources and vegetation indices.