Nasutitermes tiantongensis belongs to Nasutitermitinae and its mitochondrial genome was determined in this study. It consisted of 13 PCGs, 22 tRNAs, 2 rRNAs, and an A + T-rich control region, and its length was 15824 bp. The phylogenetic analysis suggested that the genus Nasutitermes was not monophyletic, and N. tiantongensis formed a sister group with Bultitermes laticephalus. The mitochondrial genome of N. tiantongensis provides a resource for evolutionary analysis within Nasutitermitinae.