A method with exceptional comprehensive performance for gold nanorod synthesis using 3-aminophenol as the reducing agent.

No abstract

Biothiols, a vital branch of reactive sulfur species (RSS) family, are indispensable in human physiology. However, the exact functional roles of each biothiol involved in complicated physiological activities are still not fully clarified. A critical barrier is a lack of robust molecular tools which can simultaneously visualize different biothiols with distinct emission signals. Herein, the first lysosome-targetable fluorescent probe, Lyso-RC, which could respond to Cys/ Hcy, GSH, and H 2 S with different sets of signal patterns was developed. Lyso-RC responds to Cys/Hcy, GSH, and H 2 S with the fluorescence signal patterns of blue-red, green-red, and red, respectively. Significantly, Lyso-RC is capable of discriminating lysosomal Cys/Hcy, GSH, and H 2 S in HeLa cells.

A ratiometric nanosensor based on fluorescent carbon dots for label-free and highly selective recognition of DNA.

Biothiols are essential reactive sulfur species (RSS), which play crucial roles in various critical physiological activities. To clarify their complex correlations in signal transduction and metabolism pathways, methods to distinguish H 2 S, cysteine (Cys), homocysteine (Hcy), and glutathione (GSH) at the same time are highly needed. Herein, we deliberately integrate 7-diethylcoumarin and resorufin through an ether bond to develop RC for simultaneous differentiation of Cys, Hcy, GSH, and H 2 S for the first time. RC displays distinct fluorescence colors in response to H 2 S, Cys, Hcy, and GSH as red, blue-red, bluegreen-red, and green-red, respectively, both in aqueous media and living cells. This work not only reported a robust molecule, RC, to disentangle the intricate interplay networks among different biothiols in biosystems but also demonstrated a strategy for the design of multisignal fluorescent probes for the simultaneous sensing of diverse RSS species as well as other biological species.