Chromate (Cr(VI)), as one of ubiquitous
contaminants in groundwater,
has posed a major threat to public health and ecological environment.
Although various electron donors (e.g., organic carbon, hydrogen,
and methane) have been proposed to drive chromate removal from contaminated
water, little is known for microbial chromate reduction coupled to
elemental sulfur (S(0)) or zerovalent iron (Fe(0)) oxidation. This
study demonstrated chromate could be biologically reduced by using
S(0) or Fe(0) as inorganic electron donor. After 60-day cultivation,
the sludge achieved a high Cr(VI) removal efficiency of 92.9 ±
1.1% and 98.1 ± 1.2% in two independent systems with S(0) or
Fe(0) as the sole electron donor, respectively. The deposited Cr(III)
was identified as the main reduction product based on X-ray photoelectron
spectroscopy. High-throughput 16S rRNA gene sequencing indicated that
Cr(VI) reduction coupled to S(0) or Fe(0) oxidation was mediated synergically
by a microbial consortia. In such the consortia, S(0)- or Fe(0)-oxidizing
bacteria (e.g., Thiobacillus or Ferrovibrio) could generate volatile fatty acids as metabolites, which were
further utilized by chromate-reducing bacteria (e.g., Geobacter or Desulfovibrio) to reduce chromate. Our findings
advance our understanding on microbial chromate reduction supported
by solid electron donors and also offer a promising process for groundwater
remediation.
Vanadium mining activities can cause contamination of the surrounding geological environment. Vanadium may exist in multiple matrices due to its migration and transformation, forming interactive relationships; however, the connection between vanadium distributions in multiple matrices and microbial community responses remains largely unknown. Vanadium is a redox‐sensitive metal that can be microbiologically reduced and immobilized. To date, bioremediation of vanadium‐contaminated environments by indigenous microorganisms has rarely been evaluated. This paper reports a systematic investigation into vanadium distributions and microbial communities in soils, water, and sediment from Panzhihua, China. Large vanadium contents of 1130.1 ± 9.8 mg/kg and 0.13 ± 0.02 mg/L were found in surface soil and groundwater. Vanadium in surface water tended to precipitate. Microbial communities isolated from similar environments were alike due to similarity in matrix chemistry whereas communities were distinct when compared to different matrices, with lower richness and diversity in groundwater. Proteobacteria was distributed widely and dominated microbial communities within groundwater. Redundancy analysis shows that vanadium and nutrients significantly affected metal‐tolerant bacteria. Long‐term cultivation (240 days) suggests the possibility of vanadium bioremediation by indigenous microorganisms, within acid‐soluble fraction. This active fraction can potentially release mobile vanadium with shifted redox conditions. Vanadium (V) was bio‐reduced to less toxic, mobile vanadium (IV) primarily by enriched Bacillus and Thauera. This study reveals the biogeochemical fate of vanadium in regional geological environments and suggests a bioremediation pathway via native vanadium‐reducing microbes.
Groundwater co-contaminated by vanadium (V) (V(V)) and nitrate requires efficient remediation to prevent adverse environmental impacts. However, little is known about simultaneous bio-reductions of V(V) and nitrate supported by gaseous electron donors in aquifers. This study is among the first to examine microbial V(V) reduction and denitrification with hydrogen as the sole electron donor. V(V) removal efficiency of 91.0 ± 3.2% was achieved in test bioreactors within 7 d, with synchronous, complete removal of nitrate. V(V) was reduced to V(IV), which precipitated naturally under near-neutral conditions, and nitrate tended to be converted to nitrogen, both of which processes helped to purify the groundwater. Volatile fatty acids (VFAs) were produced from hydrogen oxidation. High-throughput 16S rRNA gene sequencing and metagenomic analyses revealed the evolutionary behavior of microbial communities and functional genes. The genera Dechloromonas and Hydrogenophaga promoted bio-reductions of V(V) and nitrate directly coupled to hydrogen oxidation. Enriched Geobacter and denitrifiers also indicated synergistic mechanism, with VFAs acting as organic carbon sources for heterotrophically functional bacteria while reducing V(V) and nitrate. These findings are likely to be useful in revealing biogeochemical fates of V(V) and nitrate in aquifer and developing technology for removing them simultaneously from groundwater.
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