Fungal infections result in decreases in photosynthesis, induction of stress and signaling volatile emissions and reductions in constitutive volatile emissions, but the way different physiological processes scale with the severity of infection is poorly known. We studied the effects of infection by the obligate biotrophic fungal pathogen Melampsora larici-populina Kleb., the causal agent of poplar leaf rust disease, on photosynthetic characteristics, and constitutive isoprene and induced volatile emissions in leaves of Populus balsamifera var. suaveolens (Fisch.) Loudon. exhibiting different degrees of damage. The degree of fungal damage, quantified by the total area of chlorotic and necrotic leaf areas, varied between 0 (noninfected control) and ∼60%. The rates of all physiological processes scaled quantitatively with the degree of visual damage, but the scaling with damage severity was weaker for photosynthetic characteristics than for constitutive and induced volatile release. Over the whole range of damage severity, the net assimilation rate per area (AA) decreased 1.5-fold, dry mass per unit area 2.4-fold and constitutive isoprene emissions 5-fold, while stomatal conductance increased 1.9-fold and dark respiration rate 1.6-fold. The emissions of key stress and signaling volatiles (methanol, green leaf volatiles, monoterpenes, sesquiterpenes and methyl salicylate) were in most cases nondetectable in noninfested leaves, and increased strongly with increasing the spread of infection. The moderate reduction in AA resulted from the loss of photosynthetically active biomass, but the reduction in constitutive isoprene emissions and the increase in induced volatile emissions primarily reflected changes in the activities of corresponding biochemical pathways. Although all physiological alterations in fungal-infected leaves occurred in a stress severity-dependent manner, modifications in primary and secondary metabolic pathways scaled differently due to contrasting operational mechanisms.

Release of stress volatiles—methanol, lipoxygenase pathway compounds, and monoterpenes and sesquiterpenes—from cucumber leaves upon exposure to methyl jasmonate (MeJA) is biphasic and MeJA dose dependent.

Oak trees (Quercus) are hosts of diverse gall-inducing parasites, but the effects of gall formation on the physiology and biochemistry on host oak leaves is poorly understood. The influence of infection by four species from two widespread gall wasp genera, Neuroterus (N. anthracinus and N. albipes) and Cynips (C. divisa and C. quercusfolii), on foliage morphology, chemistry, photosynthetic characteristics, constitutive isoprene, and induced volatile emissions in Q. robur was investigated. Leaf dry mass per unit area (M A ), net assimilation rate per area (A A ), stomatal conductance (g s ), and constitutive isoprene emissions decreased with the severity of infection by all gall wasp species. The reduction in A A was mainly determined by reduced M A and to a lower extent by lower content of leaf nitrogen and phosphorus in gall-infected leaves. The emissions of lipoxygenase pathway volatiles increased strongly with increasing infection severity for all 4 species with the strongest emissions in major vein associated species, N. anthracinus. Monoterpene and sesquiterpene emissions were strongly elicited in N. albipes and Cynips species, but not in N. anthracinus. These results provide valuable information for diagnosing oak infections using ambient air volatile fingerprints and for predicting the impacts of infections on photosynthetic productivity and whole tree performance.

Past studies have focused on the composition of essential oil of leaves, but data on composition and regulation of its aerial emissions, especially floral volatile emissions are scarce. We studied the chemical profile, within-flower spatial distribution (sepals, petals, pistils with stamina and pedicels), diurnal emission kinetics and effects of exogenous methyl jasmonate (MeJA) application on the emission of floral volatiles by dynamic headspace collection and identification using gas chromatography-mass spectrometry (GC-MS) and proton transfer reaction mass spectrometry (PTR-MS). We observed more abundant floral emissions from flowers compared with leaves. Sepals were the main emitters of floral volatiles among the flower parts studied. The emissions of lipoxygenase compounds (LOX) and monoterpenoids, but not sesquiterpene emissions, displayed a diurnal variation driven by light. Response to exogenous MeJA treatment of flowers consisted of a rapid stress response and a longer-term acclimation response. The initial response was associated with enhanced emissions of fatty acid derivatives, monoterpenoids, and sesquiterpenoids without variation of the composition of individual compounds. The longer-term response was associated with enhanced monoterpenoid and sesquiterpenoid emissions with profound changes in the emission spectrum. According to correlated patterns of terpenoid emission changes upon stress, highlighted by a hierarchical cluster analysis, candidate terpenoid synthases responsible for observed diversity and complexity of released terpenoid blends were postulated. We conclude that flower volatile emissions differ quantitatively and qualitatively from leaf emissions, and overall contribute importantly to flavor, especially under stress conditions.

Poplar spiral gall aphid (Pemphigus spyrothecae) forms galls on the petiole in poplars (Populus) and mass infestations are frequent in poplar stands, but how these parasite gall infestations can affect the leaf lamina structure, photosynthetic rate and constitutive and stress volatile emissions is unknown. We investigated how the infestation by the petiole gall aphids affects lamina photosynthetic characteristics (net assimilation rate, stomatal conductance), C and N contents, and constitutive isoprene and induced volatile emissions in Populus × petrovskiana. The dry gall mass per leaf dry mass (Mg/Ml) was used as a quantitative measure of the severity of gall infestation. Very high fraction of leaf biomass was invested in gall formation with Mg/Ml varying between 0.5-2. Over the whole range of the infestation severities, net assimilation rate per area, leaf dry mass per unit area and N content decreased with increasing the severity of infestation. In contrast, stomatal conductance, leaf dry mass per fresh mass, constitutive isoprene emissions, and induced green leaf volatile (GLV), monoterpene, sesquiterpene and benzenoid emissions increased with increasing the severity of gall infestation. The rates of induced emissions were low and these emissions were associated with methyl jasmonate release from leaf laminas. The data demonstrate that petiole gall infestations lead to major changes in leaf lamina sink-source relationships and leaf water relations, thereby significantly altering lamina photosynthesis. Modifications in stress-induced emissions likely indicated systemic signaling triggered by jasmonate transported from the petiole galls to the lamina where jasmonate elicited a cascade of volatile emission responses. Enhance isoprene emissions and induced volatile emissions can play a major role in indirect defense against other herbivores, securing the food source for the gall aphids. In conclusion, a massive infestation by petiole gall aphids can profoundly modify the foliage photosynthetic performance and volatile emission profiles in poplars.