Background: Vps4 ATPase is stimulated by the interaction between Vta1 and Vps60, but the structural basis for this interaction remains unclear. Results: The structure of the Vta1 N-terminal domain (Vta1NTD) in complex with Vps60(128 -186) was determined. Conclusion: Vps60(128 -186) interacts with Vta1NTD through helices ␣4Ј and ␣5Ј, extending over Vta1NTD MIT2 domain helices 1-3. Significance: This is a novel MIT recognition mode.
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