A survey was conducted in 2000 across 38 counties in Mississippi on 192 randomly selected soybean fields to assess the most common occurring weeds. Statewide, prickly sida, which was present in 40% of the fields sampled, was the most common. Pitted and entireleaf morningglory were present in 34 and 29% of the soybean fields, respectively. Broadleaf signalgrass and barnyardgrass were the most common annual grasses, and yellow nutsedge was the most common sedge observed. Trumpetcreeper and redvine were the most common perennial vines. In the Mississippi Delta region of Mississippi, prickly sida was present in 45% of the fields sampled. The trend of occurrence of other species in the Delta mirrored statewide results. In eastern Mississippi, prickly sida and broadleaf signalgrass were found in 43% of soybean fields. Sicklepod, common cocklebur, and balloonvine were more prevalent in eastern Mississippi, when compared with the Mississippi Delta. Since 1982, there has been a sevenfold decline in the occurrence of common cocklebur and a fourfold decline in the occurrence of johnsongrass in Mississippi soybean. Also, the occurrences of redroot pigweed, common ragweed, and fall panicum have declined. Conversely, the occurrences of yellow nutsedge and broadleaf signalgrass have increased. The occurrences of barnyardgrass, prickly sida, redvine and trumpetcreeper have been relatively static over the past two decades.

When the incorporation of tritiated thymidine into acid insoluble material was measured, ribavirin appeared to be a potent inhibitor of DNA synthesis in KB cells and human lymphocytes. Inhibition was nearly 100-fold less, however, when DNA synthesis was measured by incorporation of phosphorus-32-labeled phosphate or by DNA fluorescence. The potent inhibition detected by incorporation of tritiated thymidine into DNA actually was the result of a potent effect on the labeling of deoxythymidine triphosphate, not on the synthesis of DNA.

No abstract

No abstract

Defective herpes simplex virus type 1 genomes are composed of head-to-tail tandem repeats of small regions of the nondefective genome. Monomeric repeat units of class I defective herpes simplex virus genomes were cloned into bacterial plasmids. The repeat units functioned as replicons since both viral and convalently linked bacterial plasmid DNA replicated (with the help of DNA from nondefective virus) when transfected into rabbit skin cells. Recombinant plasmids were packaged into virions and were propagated from culture to culture by infection with progeny virus. Replication was evidently by a rolling circle mechanism since plasmid DNA was present in a high-molecular-weight form in transfected cells. Circular recombinant plasmid DNA replicated with a high degree of fidelity. In contrast, linear plasmid DNA underwent extensive deletions of both viral and bacterial sequences when transfected into rabbit skin cells. Derivative plasmids, a fraction of the size of the parental plasmid, were rescued by transforming Escherichia coli with DNA from the transfected rabbit skin cells. These plasmids functioned as shuttle vectors since they replicated faithfully in both eucaryotic and procaryotic cells.