cryopreservation of cord blood hematopoetic stem cells. The recovered cells' viability was tested by typan blue staining, and the effect of EG group was best. The same result get from the recovery rate tested by MNC counted and receiver rate of CFU-GM. The result of the test of CFU-S showed that the effect of EG was better than DMSO (P < 0.05) and the diameter of CFU-S in EG group was the highest (P < 0.05), either about the WBC count in the peripheral blood of mouse (P < 0.05). These result suggest that the effects of protection were best when EG was composed of 1.5 M EG+ 0.25 M Suc + 1% Dex-40 in the vitrification cryopreservation of hematopoetic stem cells.In order to explore the chemotherapeutic sensitivity of brain tumor stem cells (BTSCs), we obtained fresh specimens of astrocytoma and then cultivated them primarily into BTSCs and brain tumor cells (BTCs). By drug S24Abstracts / Cell Biology International 32 (2008) S1eS67