In hospitals, Acinetobacter baumannii is considered one of the major pathogens due to its antibiotic and antiseptic resistance capacity thus it persists in a stressed environment and leads to nosocomial infections. Its persistence is influenced by biofilm development. This study looked at 60 A. baumannii isolates from four Iraqi hospitals. A significant distribution of biofilm-forming characteristics was identified among A. baumannii isolates obtained from several hospitals in this research. They determined the antibiotic sensitivity pattern of A. baumannii towards 13 different antibiotics (Imipenem, Meropenem, Colistin Sulfate, Doripenem, Ceftazidime, Cefepime, Cefiderocol, Amikacin, Levofloxacin, Tobramycin, Ciprofloxacin, Gentamicin, and Piperacillin). The frequency of multidrug resistance (MDR) was found to be 58.3 % in the tested strains, while the frequency of extensively drug resistance (XDR) was found to be 31.6 %. 100% of the clinical isolates could form biofilms, with 6.6 % having weak biofilm formation ability, 86.6 % having moderate biofilm formation ability, and 6.6 %having high biofilm formation capacity.