Aiming at the problem of the low removal efficiency of biological nitrogen-removing of low temperature waste-water, using Polyurethane Porous Gel Carrier (PPGC)-SBMBBR treated low temperature sewage, in compared with conventional SBR,and viaing Miseq high-throughput sequencing technology in analysis of the differences of microbial diversity and abundance of structure on the two reactors of activated sludge, revealed dominant nitrogen-removing bacterium improving the treatment efficiency of low temperature sewage. The results shows that the removal efficiency of the effluent nitrogen and the sludge sedimentation rate of (PPGC)-SBMBBR reactor are significantly improved under the water temperature (6.5±1℃). Adding the filler can contribute to improvement of bacterial diversity and relative abundance of nitrification and denitrification bacterium in the activated sludge system. The main relative abundance of ammonia oxidizing bacteria (AOB),nitrite oxidizing bacteria (NOB),anaerobic denitrifying bacteria, and aerobic denitrifying bacteria in (PPGC)-SBMBBR(R2) are significantly better than SBR (R1),and the R2 reactor can independently enrich the nitrifying bacteria and the aerobic denitrifying bacteria, such as Nitrospira, Hydrogens, Pseudomonas, and Zoogloea. The total relative abundance of dominant and nitrifying denitrifying bacterium increases from 28.65% of R1 to 60.23% of R2, providing a microbiological reference for improving the efficiency of biological nitrogen removal in low temperature waste-water.E3S Web of Conferences 158, 04002 (2020)

Based on the establishment of a stable anaerobic ammonia oxidation treatment system in 100 days, the impact resistances of two different anammox fiber fillers (the curtain filler: R1 and the bundle filler: BR) were compared.

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The article investigated the effect of substrate ratio and shock load resistance on the nitrogen removal efficiency of curtain packing anammox filler and bundle packing anammox filler. The results had shown that the nitrogen removal performance was recommendable when the NH4 +-N: NO2 −-N was set as 1:1.20, regardless of the curtain packing filler or the bundle packing filler. After the total nitrogen concentration was directly increased from 770 mg/L to 1320 mg/L for 12 h. From the perspective of the steady of nitrogen removal performance, the bundle packing filler was better than curtain packing filler, and the effluent TN concentration was beyond 70 mg/L. Subsequently, within 1 day when the influent TN concentration increased to 770 mg/L, both filler systems quickly recovered. The structure of the bundle packing was beneficial to the fixed, cultured, and aggregation of anammox bacteria made it a better filler type. The high-throughput results showed that the dominant bacteria in the two packing fillers was Candidatus Kuenenia, which accounted for 15.16% and 22.85% in the curtain packing filler and the bundle packing filler system respectively.