Cell death can be classified into two categories: apoptosis and necrosis. Apoptotic pathway can be either caspase-dependent or caspase-independent. Caspase-independent cytopathic effect (CPE) has been described. In order to evaluate the pattern of HeLa cell death induced by Coxsackievirus B3 (CVB3) and whether apoptosis involves caspase activation, we co-cultivated HeLa cells with CVB3 and detected the cytopathic changes, the alteration of mRNA and protein expression of caspase-3 gene plus caspase-3 activity, as well as analyzing DNA fragmentation before and after caspase-3 activity inhibition. According to the results, we propose that CVB3 may induce apoptosis and necrosis in HeLa cells, the latter appearing much earlier. Caspase-3 is activated at the levels of both transcription and translation, and procaspase-3 is proteolytically cleaved, thus leading to the continuous increasing of both caspase-3 precursor protein and its subunit. However, besides CPE, apoptosis induced by CVB3 is not a direct consequence of the activation of caspase-3, or caspase-3 is not the only effector molecule in apoptotic cell death, for caspase-3 inhibitor can not decrease DNA fragmentation. Some other biochemical mechanisms may participate in the process, whose role weakens the effect of inhibiting caspase-3 activity.

As cloud applications proliferate and data-processing demands increase, server resources must grow to unleash the performance of emerging workloads that scale well with large number of compute nodes. Nevertheless, power has become a crucial bottleneck that restricts horizontal scaling (scale out) of server systems, especially in datacenters that employ power over-subscription. When a datacenter hits the maximum capacity of its power provisioning equipment, the owner has to either build another facility or upgrade existing utility power infrastructure -both approaches add huge capital expenditure, require significant construction lead time, and can further increase the owner's carbon footprint.This paper proposes Oasis, a power provisioning scheme for enabling power-/carbon-constrained datacenter servers to scale out economically and sustainably. Oasis naturally supports incremental power capacity expansion with near-zero environmental impact as it takes advantages of modular renewable energy system and emerging distributed battery architecture. It allows scale-out datacenter to double its capacity using 100% green energy with up to 25% less overhead cost. This paper also describes our implementation of Oasis prototype and introduces our multi-source driven power management scheme Ozone. Ozone allows Oasis to identify the most suitable power supply control strategies and adjust server load cooperatively to maximize overall system efficiency and reliability. Our results show that Ozone could reduce the performance degradation of Oasis to 1%, extend Oasis battery lifetime by over 50%, and almost triple the average battery backup capacity which is crucial for mission-critical systems. Figure 1: "How will your organization handle increased capacity demand over the next 12-18 months" -results from the Uptime Institute 2012 Data Center Industry Survey [3].

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The motility and chemotaxis systems are critical for the virulence of leptospires. In this study, the phylogenetic profiles method was used to predict the interaction of chemotaxis proteins. It was shown that CheW1 links to CheA1, CheY, CheB and CheW2; CheW3 links to CheA2, MCP (LA2426), CheB3 and CheD1; and CheW2 links only to CheW1. The similarity analysis demonstrated that CheW2 of Leptospira interrogans strain Lai had poor homology with CheW of Escherichia coli in the region of residues 30-50. In order to verify the function of these proteins, the putative cheW genes were cloned into pQE31 vector and expressed in wild-type E. coli strain RP437 or cheW defective strain RP4606. The swarming results indicated that CheW1 and CheW3 could restore swarming of RP4606 while CheW2 could not. Overexpression of CheW1 and CheW3 in RP437 inhibited the swarming of RP437, whereas the inhibitory effect of CheW2 was much lower. Therefore, we presumed that CheW1 and CheW3 might have the function of CheW while CheW2 does not. The existence of multiple copies of chemotaxis homologue genes suggested that L. interrogans strain Lai might have a more complex chemosensory pathway.

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