TR3, an immediate-early response gene and an orphan member of the steroid-thyroid hormone-retinoid receptor superfamily of transcription factors, regulates apoptosis through an unknown mechanism. In response to apoptotic stimuli, TR3 translocates from the nucleus to mitochondria to induce cytochrome c release and apoptosis. Mitochondrial targeting of TR3, but not its DNA binding and transactivation, is essential for its proapoptotic effect. Our results reveal a mechanism by which a nuclear transcription factor translocates to mitochondria to initiate apoptosis.

BackgroundBacteriophage could be an alternative to conventional antibiotic therapy against multidrug-resistant bacteria. However, the emergence of resistant variants after phage treatment limited its therapeutic application.Methodology/Principal FindingsIn this study, an approach, named “Step-by-Step” (SBS), has been established. This method takes advantage of the occurrence of phage-resistant bacteria variants and ensures that phages lytic for wild-type strain and its phage-resistant variants are selected. A phage cocktail lytic for Klebsiella pneumoniae was established by the SBS method. This phage cocktail consisted of three phages (GH-K1, GH-K2 and GH-K3) which have different but overlapping host strains. Several phage-resistant variants of Klebsiella pneumoniae were isolated after different phages treatments. The virulence of these variants was much weaker [minimal lethal doses (MLD)>1.3×109 cfu/mouse] than that of wild-type K7 countpart (MLD = 2.5×103 cfu/mouse). Compared with any single phage, the phage cocktail significantly reduced the mutation frequency of Klebsiella pneumoniae and effectively rescued Klebsiella pneumoniae bacteremia in a murine K7 strain challenge model. The minimal protective dose (MPD) of the phage cocktail which was sufficient to protect bacteremic mice from lethal K7 infection was only 3.0×104 pfu, significantly smaller (p<0.01) than that of single monophage. Moreover, a delayed administration of this phage cocktail was still effective in protection against K7 challenge.Conclusions/SignificanceOur data showed that the phage cocktail was more effective in reducing bacterial mutation frequency and in the rescue of murine bacteremia than monophage suggesting that phage cocktail established by SBS method has great therapeutic potential for multidrug-resistant bacteria infection.

The retinoblastoma protein-interacting zinc finger gene RIZ (PRDM2) is a member, by sequence homology, of a nuclear protein-methyltransferase (MTase) superfamily involved in chromatin-mediated gene expression. The gene produces two protein products, RIZ1 that contains a conserved MTase domain and RIZ2 that lacks the domain. RIZ1 gene expression is frequently silenced in human cancers, and the gene is also a common target of frameshift mutation in microsatellite-unstable cancers. We now report studies of mice with a targeted mutation in the RIZ1 locus. The mutation inactivates RIZ1 but not RIZ2. These RIZ1 mutant mice were viable and fertile but showed a high incidence of diffuse large B-cell lymphomas (DLBL) and a broad spectrum of unusual tumors. RIZ1 deficiency also accelerated tumorigenesis in p53 heterozygous mutant mice. Finally, several missense mutations of RIZ1 were found in human tumor tissues and cell lines; one of these was particularly common in human DLBL tumors. These missense mutations, as well as the previously described frameshift mutation, all mapped to the MTase functional domains. All abolished the capacity of RIZ1 to enhance estrogen receptor activation of transcription. These data suggest a direct link between tumor formation and the MTase domain of RIZ1 and describe for the first time a tumor susceptibility gene among methyltransferases.

Phage-coded lysin is an enzyme that destroys the cell walls of bacteria. Phage lysin could be an alternative to conventional antibiotic therapy against pathogens that are resistant to multiple antibiotics. In this study, a novel staphylococcal phage, GH15, was isolated, and the endogenous lytic enzyme (LysGH15) was expressed and purified. The lysin LysGH15 displayed a broad lytic spectrum; in vitro treatment killed a number of Staphylococcus aureus strains rapidly and completely, including methicillinresistant S. aureus (MRSA). In animal experiments, a single intraperitoneal injection of LysGH15 (50 g) administered 1 h after MRSA injections at double the minimum lethal dose was sufficient to protect mice (P < 0.01). Bacteremia in unprotected mice reached colony counts of about 10 7 CFU/ml within 3.5 h after challenge, whereas the mean colony count in lysin-protected mice was less than 10 4 CFU/ml (and ultimately became undetectable). These results indicate that LysGH15 can kill S. aureus in vitro and can protect mice efficiently from bacteremia in vivo. The phage lysin LysGH15 might be an alternative treatment strategy for infections caused by MRSA.Staphylococcus aureus is a common and dangerous pathogen that causes various infectious diseases, including skin abscesses, wound infections, endocarditis, osteomyelitis, pneumonia, and toxic shock syndrome (2, 23). Treatment of these infections has become ever more difficult due to the emergence of multidrug-resistant strains, especially methicillin-resistant S. aureus (MRSA) (15,25,26,36,37). Vancomycin was effective against MRSA, but certain MRSA strains have already acquired resistance to vancomycin as well (vancomycin-resistant S. aureus [VRSA]), raising serious concerns within the medical community (17,18,37). Therefore, there is an urgent need for novel therapeutic agents directed against this formidable pathogen (2, 9).The phage lysin is encoded by the bacteriophage genome and is synthesized at the end of the phage lytic life cycle to lyse the host cell (30). Lysins belong to the family of mureolytic enzymes that directly destroy peptidoglycans in the bacterial cell wall. Previous studies have suggested that lysins from certain phages were highly efficient in lysing bacteria, especially when applied exogenously (11,14,21,22,29,35). As a potential antibacterial agent, lysins possess several promising features, namely, a distinct mode of action, species or type specificity, and bactericidal activity independent of the antibiotic susceptibility pattern (1). Indeed, there is a low probability that bacteria will develop resistance against lysin (12, 21).Some Staphylococcus phage lysins have been isolated and studied, including LysK, ClyS, MV-L, LysWMY, and ⌽H5; however, only MV-L and ClyS have been studied in in vivo assays (6, 33). In this study, a novel myovirus phage infecting S. aureus was isolated. The lysin derived from this phage, LysGH15, was expressed and refined. The lysin LysGH15 demonstrated a very broad host range and strong lytic activity. We evaluate...