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UAS captured increased genetic variation compared with manual terminal height. There were small significant differences in ground filtering methods to extract plant structure. Higher resolution did not improve imagery informativeness with regard to plant height. Logistic function provides informative phenotypes for temporal maize growth. Correlation and prediction accuracy of grain yield increased by ∼20% with UAS heights. Weekly unmanned aerial system (UAS) imagery was collected over the College Station, TX, 2017 Genomes to Fields (G2F) hybrid trial, across three environmental stress treatments, using two UAS platforms. The high‐altitude (120‐m) fixed‐wing platform increased the fraction of variation attributed to genetics and had highly repeatable (R > 60%) height estimates, increasing the genetic variance explained (10–40%) over traditional terminal plant height measurement (PHTTRML ∼30%), as well as over the low‐altitude rotary‐wing UAS platform (10–20%). A logistic function reduced the dimensionality (>20 flights) of each UAS dataset to three parameters (inflection point, growth rate, and asymptote) and produced a more robust predictive model than independent flight dates, effectively summarizing (R2 > 0.98) the UAS flight dates. The logistic model overcame the need to use specific flight dates when comparing different environments. The UAS height estimates (r = 0.36–0.48) doubled the correlations to grain yield in this G2F experiment compared with PHTTRML (r = 0.23–0.28). Parameters of the logistical function achieved equivalent correlations (r = 0.30–0.46) to individual flight dates (r = 0.36–0.48), improving grain yield prediction by ∼400% (R2 = 0.25–0.34) over PHTTRML (R2 = 0.06–0.08). Incorporating other UAS‐derived parameters beyond plant height may allow yield to be accurately predicted before maturity, speeding breeding programs. A new public R function to generate ESRI shapefiles for plot research is also described.

Core Ideas We comprehensively validated the use of UAS in sorghum and maize breeding programs. Temporal estimates of plant growth will allow researchers to elucidate new phenotypes. The stage of the breeding pipeline dictates the applicability of UAS platforms. The implementation of UAS is demonstrated in different crop species. Monetary and time costs should be considered before implementation of UAS. To meet future world food and fiber demands, plant breeders must increase the rate of genetic improvement of important agricultural crops. One of the biggest obstacles now facing crop scientists is a phenotyping bottleneck. To ease this burden, the emerging technology of unmanned aerial systems (UAS) presents an exciting opportunity. To assess the utility of UAS, it is important to investigate their application across multiple crop species. Terminal plant height is of great importance to maize (Zea mays L.) and sorghum [Sorghum bicolor (L.) Moench] breeders and has been hypothesized to be useful but has been logistically impractical to measure in the field. In this study, we statistically analyzed in depth the ability of UAS to estimate height in sorghum (advanced and early generation material) and maize (optimal and late material) and the application of these estimates in breeding programs. We found that UAS explain genotypic variation similarly to ground‐truth methods and that the repeatability of the methodology is high (R = 0.61–0.99), indicating effective differentiation of genotypes. Additionally, correlations between ground truth and UAS measurements were moderate to high for all materials (r = 0.4–0.9). Finally, we present a novel application for the technology in the form of high‐resolution temporal growth curves. Using these UAS‐generated growth curves, new physiological insights can be obtained and new avenues of scientific investigation are possible.