Melibiose is widely used as a functional carbohydrate. Whole-cell biocatalytic production of melibiose from raffinose could reduce its cost. However, characteristics of strains for whole-cell biocatalysis and mechanism of such process are unclear. We compared three different Saccharomyces cerevisiae strains (liquor, wine, and baker's yeasts) in terms of concentration variations of substrate (raffinose), target product (melibiose), and by-products (fructose and galactose) in whole-cell biocatalysis process. Distinct difference was observed in whole-cell catalytic efficiency among three strains. Furthermore, activities of key enzymes (invertase, α-galactosidase, and fructose transporter) involved in process and expression levels of their coding genes (suc2, mel1, and fsy1) were investigated. Conservation of key genes in S. cerevisiae strains was also evaluated. Results show that whole-cell catalytic efficiency of S. cerevisiae in the raffinose substrate was closely related to activity of key enzymes and expression of their coding genes. Finally, we summarized characteristics of producing strain that offered advantages, as well as contributions of key genes to excellent strains. Furthermore, we presented a dynamic mechanism model to achieve some mechanism insight for this whole-cell biocatalytic process. This pioneering study should contribute to improvement of whole-cell biocatalytic production of melibiose from raffinose.

BACKGROUND: Aflatoxin B 1 (AFB 1 ) is the most toxic and harmful aflatoxin, and has good thermal stability. Research reveals that AFB 1 also exists in water, which is potentially harmful to human health. Therefore, it is urgent to remove AFB 1 from water. In this work, a novel magnetic covalent organic framework composite (Fe 3 O 4 @COFs) is synthesized. RESULTS:The specific surface area is as high as 1293.32 m 2 g −1 . The adsorption capacity for AFB 1 is 420 ∼g mg −1 , which is six times or more that of other adsorbents. Fe 3 O 4 @COFs is employed for the adsorption and elimination of AFB 1 from water. The removal efficiency is above 90% and it can be reused at least eight times. In addition, Fe 3 O 4 @COFs has been successfully used to remove trace AFB 1 in actual water samples.CONCLUSIONS: The synthesized Fe 3 O 4 @COFs has good thermal stability, fast adsorption and large adsorption capacity, and can be recycled many times. Fe 3 O 4 @COFs exhibits excellent performance for the removal of AFB 1 , and has exceptional promise for application in water purification.

We developed a new ochratoxin A (OTA) aptamer biosensor to promptly detect OTA in food. Mesoporous silica nanoparticles (MSN) were used as carriers, and aptamers were used as recognition probes and gating molecules. The fluorescent dye Rhodamine 6G (Rh6G) was loaded into mesoporous silica, and through electrostatic contact, the OTA aptamer was adsorbed on amino-modified mesoporous silica. The fluorescent dye released from the mesopore in the presence of OTA because of the conformational change induced in the aptamer by the target. The amount of ochratoxin was determined by measuring the fluorescence intensity. Our findings revealed a positive relationship between the fluorescence intensity and OTA concentration, with a limit of detection of 0.28 ng mL−1, and the detection range was 0.05–200 ng mL−1. The recovery rate was 80.7%–110.8% in real samples. The proposed approach is suitable for the quantification of other toxins.

Antibiotic drug residues can adversely affect the human body. Lincomycin is a common veterinary drug that can form residues in foods of animal origin. However, the detection of trace residue levels of lincomycin residues in real samples is challenging. Here, a simple solid phase extraction (SPE) method was developed for the enrichment of lincomycin from cow milk samples before its detection by high-performance liquid chromatography-tandem mass spectrometry (HPLC-MS/MS). The adsorbent used in the SPE was a Cu-based metal-organic framework (Cu-MOF) prepared by the solvothermal synthesis approach. The prepared MOFs were characterized using scanning electron microscopy (SEM), Fourier-transform infrared spectroscopy (FT-IR), X-ray diffractometry (XRD), differential thermogravimetric analysis (TG-DTA), and N2 adsorption-desorption experiments. The adsorption capacity (adsorption equilibrium, extraction time, pH), and elution solvent parameters were investigated. Under the optimized conditions of the HPLC-MS/MS method, lincomycin was detected in the linear range of 10–200 g/L with a detection limit of 0.013 ng/mL. Commercial milk samples were spiked with lincomycin, and a recovery rate between 92.3% and 97.2% was achieved. Therefore, the current method can be successfully applied for the enrichment and determination of lincomycin from milk samples.