The Lie theorem for Birkhoffian systems with time-scale nonshifted variational problems are studied, including free Birkhoffian system, generalized Birkhoffian system and constrained Birkhoffian system. First, the time-scale nonshifted generalized Pfaff-Birkhoff principle is established, and the dynamical equations for three Birkhoffian systems under nonshifted variational problems are deduced. Afterwards, in the time-scale nonshifted variational problems, by introducing the infinitesimal transformations, Lie symmetry for free Birkhoffian system, generalized Birkhoffian system and constrained Birkhoffian system are defined respectively. Then Lie symmetry theorems for three kinds of Birkhoffian systems are deduced and proved. In the end, three examples are given to explain the applications for the results.