BackgroundClear cell renal cell carcinoma (ccRCC) is one of the most common tumors in the world and affects human health seriously. PIMREG is a mitotic regulator which is essential to the metaphase-to-anaphase transition in cell cycle. Although PIMREG plays a crucial role in the malignant progression of tumors, there are few reports on its role in ccRCC.MethodsThe transcriptional expression profile and clinical data of PIMREG were downloaded from TCGA database and verified by qRT-PCR. Kaplan-Meier plotter was used to analyze the effect of PIMREG on overall survival (OS), disease specific survival (DSS) and progression-free interval (PFI) of patients with ccRCC. Univariable and multivariable Cox regression analysis were used to determine the independent prognostic factors of ccRCC. The effects of PIMREG on cell migration and invasion were detected by wound healing assay and transwell invasion assay, and CCK-8 assay, colony formation assay and cell cycle assay were used to detect the effect of PIMREG on cell proliferation. In addition, the changes in cell cycle related proteins were detected by western blot.ResultsPIMREG was highly expressed in human ccRCC and was positively correlated with pathologic stage, TNM stage and histologic grade. In addition, patients with high expression of PIMREG had a poor prognosis. Univariable and multivariable Cox regression analysis identified that PIMREG was an independent prognostic factor of ccRCC. Additionally, PIMREG was also closely related to immune cell infiltration. Experiments in vitro identified that the knockdown of PIMREG could significantly inhibit the proliferation, migration and invasion abilities of ccRCC. The expression of cyclin D1, CDK4 and CDK6 was also significantly reduced after PIMREG knockdown.ConclusionsPIMREG plays a vital role in the development of ccRCC and may become a potential therapeutic target in the future.

Primary seminal vesicle tumors are extremely rare. Several rare pathological types of primary seminal vesicle tumors have been reported, such as adenocarcinoma, but there is no report on adenofibroma. We report the first case of adenofibroma arising from the seminal vesicle. A 50-year-old man, with no history or clinical evidence of any other tumors, accidentally found a pelvic mass during an ultrasound examination. As the mass grew, the patient developed mild constipation, without genitourinary or other symptoms. All laboratory examinations were normal. MRI of the pelvis revealed a mixed density, measuring 11.7×9.9×8.2cm, well circumscribed mass. The rectum, bladder, prostate and lymph nodes were normal. We successfully performed the open surgery and removed the mass. Histopathological results confirmed that the mass was a primary seminal vesicle adenofibroma. In literature, we found that for biphasic differentiated tumors, it is easy to reduce the accuracy of pathological diagnosis because of insufficient puncture. Therefore, preoperative puncture biopsy for seminal vesicle tumors should be investigated further.

Background: Kidney renal clear cell carcinoma (KIRC) is a highly prevalent and lethal type of cancer, and one of the primary approaches to address it is the development of suitable biomarkers. The objective of this study is to investigate the prognostic value of proline-rich γ-carboxyglutamic acid protein 2 (PRRG2) in KIRC and its correlation with immune cell infiltration. Methods: The study comprehensively clarified the prognostic value and potential function of PRRG2 in KIRC. PRRG2 expression was analyzed and correlated with sex, age, metastasis, and pathological stage in KIRC patients. Gene Ontology (GO), Kyoto Encyclopedia of Genes and Genomes (KEGG) and Gene Set Enrichment Analysis (GSEA) were used to explore the involvement of PRRG2 in the immune response. The correlation between PRRG2 expression and immune cell infiltration was also analyzed. Finally, the confirmation of cellular and tissue identity was achieved through the utilization of immunohistochemical staining and quantitative real-time PCR. Results: The study reveals a significant reduction in PRRG2 expression among KIRC patients. Low PRRG2 expression is associated with gender, age, metastasis, and pathological staging, and significantly predicts poor prognosis in KIRC patients. GO, KEGG, and GSEA results indicate the involvement of PRRG2 in immune response. PRRG2 expression is significantly correlated with the infiltration level of immune cells and the expression of different immune cell markers. Conclusion: PRRG2 may potentially impact prognosis in KIRC patients by regulating immune infiltration, thus rendering PRRG2 a promising candidate prognostic biomarker for KIRC-associated immune infiltration.