This study was done to examine the assessment of floral diversity of least concern (LC) plant species based on the plot quadrat sampling patterns on the accuracy of phytosociological characterization of the road side floral diversity. The size of each plot was taken as 10 m 2 . LC plant species in each quadrat along the National Highway (NH-2) Delhi-Kolkata were identified, and total 16 LC plant species were recorded in each 24 randomly selected quadrat along the both sides of National Highway. A total of 16 LC plant species belonging to 13 families and 16 genera were recorded in which family Fabaceae was represented by four species followed by Asteraceae, Apocynaceae, Amaranthaceae, Cactaceae, Pteridaceae, Dipterocarpaceae, Cupressaceae, Cyperaceae, Arecaceae, Acanthaceae, Pontederiaceae, and Convolvulaceae by only a single species. The result showed that the LC plant species had a floral diversity composed of a total of 16 LC plant species of tree, shrub, herb, climber, and cactus, respectively, with varied qualities. These research paper deals with the floristic assessment are the necessary prerequisites to understand the present diversity status of LC species along the National Highway.
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