A highly sensitive and water-soluble "switch-on" fluorescent probe with aggregation-induced emission characteristics was developed for protein quantification and visualization. It offers a rapid, economic and effective way for the assay of complete serum proteins and disease-marker proteins.

Erythropoietin (EPO) is an attractive protein-unfolding/folding model because of its high degree of unfolding and folding reversibility and intermediate size. Due to its function for regulating red blood cell production by stimulating late erythroid precursor cells, EPO presents obvious values to biological research. A nonemissive anthracene derivative, that is 9,10-bis[4-(3-sulfonatopropoxyl)-styryl]anthracene sodium salt (BSPSA), with aggregation-induced emission (AIE) charateristics shows a novel phenomenon of AIE when EPO is added. The AIE biosensor for EPO shows the limit of detection is 1 × 10(-9) M. Utilizing the AIE feature of BSPSA, the unfolding process of EPO using guanidine hydrochloride is monitored, which indicates three steps for the folding structures of EPO to transform to random coil. Computational modeling suggests that the BSPSA luminogens prefer docking in the hydrophobic cavity in the EPO folding structures, and the assembly of BSPSA in this cavity makes the AIE available, making the monitoring of unfolding of EPO possible.

The self-assembly of diphenylalanine peptides (l-Phe-l-Phe) into microtubes with "turn on" bright yellow green fluorescence was described, which was achieved using an aggregation-induced emission (AIE) molecule of 9,10-bis[4-(3-sulfonatopropoxyl)-styryl] anthracene (BSPSA) sodium.

The detection of layer-by-layer self-assembly multilayer films was carried out using low-temperature plasma (LTP) mass spectrometry (MS) under ambient conditions. These multilayer films have been prepared on quartz plates through the alternate assembling of oppositely charged 4-aminothiophenol (4-ATP) capped Au particles and thioglycolic acid (TGA) capped Ag particles. An LTP probe was used for direct desorption and ionization of chemical components on the films. Without the complicated sample preparation, the structure information of 4-ATP and TGA on films was studied by LTP-MS. Characteristic ions of 4-ATP (M) and TGA (F), including [M](+•), [M-NH(2)](+), [M-HCN-H](+), and [F + H](+), [F-H](+), [F-OH](+), [F-COOH](+) were recorded by LTP-MS on the films. However, [M-CS-H](+) and [F-SH](+) could not be observed on the film, which were detected in the neat sample. In addition, the semi-quantitative analysis of chemical components on monolayer film was carried out, and the amounts of 4-ATP and TGA on monolayer surface were 45 ng/mm(2) and 54 ng/mm(2), respectively. This resulted the ionization efficiencies of 72% for 4-ATP and 54% for TGA. In order to evaluate the reliability of present LTP-MS, the correlations between this approach and some traditional methods, such as UV-vis spectroscopy, atomic force microscope and X-ray photoelectron spectroscopy were studied, which resulted the correlation coefficients of higher than 0.9776. The results indicated that this technique can be used for analyzing the films without any pretreatment, which possesses great potential in the studies of self-assembly multilayer films.