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Background and Purpose: The promotion of hair regeneration and growth heavily depends on the activation of Wnt/β-catenin signalling in the hair follicle, including dermal papilla (DP). KY19382, one of the newly synthesized analogues of indirubin-3 0 -monoxime (I3O), was identified as a Wnt/β-catenin signalling activator via inhibition of the interaction between CXXC-type zinc finger protein 5 (CXXC5) and dishevelled (Dvl). Given the close relationship between the Wnt/β-catenin signalling and hair regeneration, we investigated the effect of KY19382 on hair regrowth and hair follicle neogenesis. Experimental Approach: In vitro hair induction effects of KY19382 were performed in human DP cells. The hair elongation effects of KY19382 were confirmed through the human hair follicle and vibrissa culture system. In vivo hair regeneration abilities of KY19382 were identified in three models: hair regrowth, wound-induced hair follicle neogenesis (WIHN) and hair patch assays using C57BL/6 mice. The hair regeneration abilities were analysed by immunoblotting, alkaline phosphatase (ALP) and immunohistochemical staining. Key Results: KY19382 activated Wnt/β-catenin signalling and elevated expression of ALP and the proliferation marker PCNA in DP cells. KY19382 also increased hair length in ex vivo-cultured mouse vibrissa and human hair follicles and induced hair regrowth in mice. Moreover, KY19382 significantly promoted the generation of de novo hair follicles as shown by WIHN and hair patch assays. Conclusion and Implications:These results indicate that KY19382 is a potential therapeutic drug that exhibits effective hair regeneration ability via activation of the Wnt/β-catenin signalling for alopecia treatments.

Background: Various sebum levels can be detected in dandruff-affected scalps. However, few studies have compared the biophysical characteristics of dandruff scalps categorized based on sebum levels. Aims: To investigate and compare the biophysical characteristics of dandruff-affected scalps categorized based on sebum levels. Methods: Fifty-four Korean women with dandruff and 30 healthy Korean women underwent physiological measurements, including evaluation of sebum and hydration levels, pH, and transepidermal water loss (TEWL) in the scalp. The levels of the biomarkers of interleukin-8 (IL-8) and kallikrein 5 (KLK5) and corneodesmosomes were investigated in the stratum corneum (SC) of the scalp. Results: Dandruff was categorized as dry (low-sebum, n = 25) or oily (high-sebum, n = 29) based on a sebum cutoff level of 97.82 μg/cm 2. Both dry and oily dandruffaffected scalps showed significantly decreased hydration levels and increased pH and TEWL compared with healthy subjects, with hydration levels being lower in dry dandruff-affected scalps. IL-8 expression was significantly increased in the oily dandruff-affected scalp. In addition, both dry and oily dandruff-affected scalps showed significantly increased KLK5 levels in the SC, with the levels being higher in oily dandruff-affected scalps. Altered distribution of corneodesmosomes, present on the entire surface area of the corneocytes, was notable in oily dandruff-affected scalps. Conclusion: The biophysical characteristics of the two types of dandruff represent the influence of different characteristics, including hydration levels, expression of IL-8 and KLK5, and corneodesmosome distribution. Thus, strategies to reduce dandruff levels should differ according to sebum levels.

We report a prediction model for sunscreen sun protection factor (SPF) and protection grade of ultraviolet (UV) A (PA) based on machine learning. We illustrate with real clinical test results of UV protection ability of sunscreen for SPF and PA. With approximately 2200 individual clinical results for both SPF and PA level detection, individually, we were able to see that active ingredient information can provide accurate SPF and PA prediction rates through machine learning. Furthermore, we included four new factors—presence of pigment, concentration of pigment grade titanium dioxide, type of formulation and type of product—as additional information for the prediction model and were able to see increased prediction rates as results.

Background and Purpose: The promotion of hair regeneration and growth heavily depends on the activation of Wnt/βcatenin signaling in the hair follicle including dermal papilla (DP). KY19382, one of the newly synthesized analogs of indirubin-3'monoxime (I3O) was identified as a Wnt/β-catenin signaling activator via inhibition of the interaction between CXXC-type zinc finger protein 5 (CXXC5) and Dishevelled (Dvl) interaction. Given the close relationship between the Wnt/β-catenin signaling and hair regeneration, we investigated the effect of KY19382 on hair re-growth and hair follicle neogenesis. Experimental Approach: In vitro hair induction effects of KY19382 was performed in human dermal papilla cells. The hair elongation effects of KY19382 was confirmed through the vibrissa culture system. In vivo hair regeneration abilities of KY19382 was identified in three models: hair regrowth, wound-induced hair follicle neogenesis (WIHN), and hair patch assays using C57BL/6 mice. The hair regeneration abilities were analyzed by immunoblotting, alkaline phosphatase (ALP) and immunohistochemical staining. Key results: KY19382 activated Wnt/β-catenin signaling and elevated the expression of ALP and proliferation marker PCNA in DP cells. KY19382 also increased hair length in ex vivo cultured mouse vibrissa follicles and induced hair regrowth in mice. Moreover, KY19382 significantly promoted the generation of de novo hair follicles as shown by WIHN and hair patch assays. Conclusion and Implications:These results indicate that KY19382 is a potential therapeutic drug that exhibits effective hair regeneration ability via activation of the Wnt/β-catenin signaling for alopecia treatments.