Glycerol, ethylene glycol, and quicklime were used as alternative dehydrating agents to obtain anhydrous bioethanol from sweet potato. The assay conditions were standardized using high bioethanol grades. For organic reagents, three volumetric ratios were studied whereas for quicklime, five concentrations were evaluated. Quicklime led to the highest bioethanol purity of 99.4°GL, followed by glycerol and ethylene glycol, reaching 96.9 and 96.6°GL, respectively. Glycerol and ethylene glycol were reused for 4 cycles with negligible loss of efficiency; however, the yield decreased by up to 8 %.