In vivo imaging of small animal models will play an increasingly important role in cancer research, as new imaging systems that employ non-invasive protocols and offer high-resolution capability become available. A flatpanel volumetric computed tomograph (fpvCT) was evaluated to determine if minimally invasive protocols can be used to provide the spatial resolution required for lung imaging in small animals. The detection of small pulmonary nodules in a Lewis carcinoma model was investigated, and fpvCT was compared with a multislice computed tomograph (MSCT). Five C57/BL6 mice with Lewis lung carcinoma were monitored with both modalities over two weeks. Sensitivity of the systems was measured by comparing the results with histology, and the incidence of first visualization of the tumors in the two systems was determined. Compared to MSCT, fpvCT proved its superior sensitivity in detection of lung nodules. Due to its isotropic resolution and a significant reduction of partial volume effects, early detection and reasonable determination of growth in very small tumors was only possible with fpvCT. fpvCT is a high-resolution imaging system that proved its ability to perform in vivo monitoring of a pulmonary lung tumor model in mice. This permits longitudinal investigations in small animals for cancer research.

In lung carcinomas the blood supply varies depending on tumor type and stage and can develop from pulmonary or bronchial circulation, or both. To examine this in vivo, primary bronchogenic Lewis lung carcinoma cells were intratracheally instilled in C57BL/6 mice. Within 7 days, histological examinations showed progressive tumor growth at the peripheral parenchymal region. The relative contribution of tumor blood supply via the pulmonary and systemic arteries was studied in detail using fluorescent microspheres (10 m). When compared to healthy lung parenchyma (13:1), Lewis lung carcinoma tumor tissue (52:1) showed a fourfold increase in pulmonary to systemic microspheres, indicating that the pulmonary arteries are the predominant tumor-feeding vessels. After filling the vessels with a vascular cast, the microanatomy of vessels being derived from the pulmonary artery was visualized with micro computed tomography. Lung cancer is among the most commonly occurring malignancies in the world and is one of the few that continues to show an increasing incidence.