Seventy‐five foods were analyzed for the vitamin pantothenic acid using a microbiological assay and a new radioimmunoassay (RIA). The food sample extract used in both assays was the result of dialysis after enzyme hydrolysis. In the method used previously, the food‐enzyme mixture had been filtered.
A very high correlation (r2=.94) between the results from the RIA and the microbiological assay was found. There was a statistically significant difference between the two assay results for all foods and for the subgroups meats, breads and cereals, and fruits and vegetables at p=.05. At p = .01 breads and cereals and fruits and vegetables did not have significantly different results between the two assay methods. For all foods and all subgroups, the microbiological assay produced a higher mean result than the RIA.
The RIA is an acceptable method for assaying pantothenic acid in breads and cereals and fruits and vegetables. Futher study is needed to determine how components of meat interact with one or both assay systems.
21 meals, representing the usual weekly dietary intake of residents of a northern Utah nursing home, were analyzed for pantothenic acid using a radioimmunoassay. Beverages were not included in the analysis. The mean daily pantothenic acid content of the diet exclusive of beverages was found to be 3.75 mg. The diet as analyzed supplied 2.22 mg of the vitamin per 1,000 kcal. If not supplemented by beverages and snacks, this diet supplies less than the 4–7 mg suggested for adults by the Food and Nutrition Board of the National Academy of Sciences.
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