The state of environmental pollution is of random character, and it depends on climatic conditions, landforms, development and industrialization. It is estimated that in the last decade as many pollutants have been released into the environment as in the previous 70 years, and the pollution rate still increases. Many scientific reports indicate that, in addition to metals, pesticides are the most commonly detected compounds in the environment. This situation is mainly due to the irrational use of these chemicals by humans. Mostly, soil environment changes caused by the influence of pesticides can be determined by various chemical analyses, which require the use of sophisticated and expensive equipment. However, biological methods, such as those using microbiological activity and an abundance of microorganisms, e.g., organisms responsible for the cycle of organic matter and nutrients, tend to be neglected. For this reason, the aim of the present study is not only to assess the validity of other research studies that were performed based on the available literature but to compile methods and compare them, which allows for an in depth understanding of the complexity of soil processes following herbicide application by conducting comprehensive soil biomonitoring.