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Longitudinal co-site optical microscopy study on the chelating ability of etidronate and EDTA using a comparative single-tooth model AbstractIn the present study the smear layer dissolution kinetics of 18% etidronate (HEBP), 9% HEBP, and 17% ethylenediaminetetraacetic acid (EDTA) on human dentin were quantitatively and longitudinally analyzed by using a single-tooth comparative model. Coronal dentin disks were prepared from 3 maxillary human molars. A standardized smear layer was produced on the pulpal side of each disk. The smear layer-covered surface was divided into 3 similar areas. Each of these was then exposed to 1 of the 3 irrigants under investigation, whereas the others were covered with adhesive tape. Co-site image sequences of the areas under investigation were obtained after several cumulative demineralization times. Sixteen images were obtained from each dentin area of each tooth for each experimental time at 1000x magnification. An image processing and analysis sequence measured sets of images, providing data of area fraction for thousands of tubules over time and allowing us to quantitatively follow the effect of the chelating substances. The Kruskal-Wallis H test and Dunn multiple comparison test were used to analyze the data. Overall, it can be concluded that the demineralization kinetics promoted by both 9% HEBP and 18% HEBP were significantly slower than those of 17% EDTA (P < .05). In addition, the single-tooth model is advantageous over the first co-site optical microscopy dentin assessments when different chelator solutions are compared. Longitudinal Co-site Optical Microscopy Study of the Chelating Ability of HEBP and EDTA Using a Comparative Single-tooth Model AbstractIn the present study the smear layer dissolution kinetics of 18% HEBP, 9% HEBP and 17%EDTA on human dentin were quantitatively and longitudinally analyzed using a singletooth comparative model. Coronal dentin disks were prepared from three maxillary human molars. A standardized smear layer was produced on the pulpal side of each disk. The smear layer-covered surface was divided into three similar areas. Each of these was then exposed to one of the three irrigants under investigation, while the others were covered with adhesive tape. Co-site image sequences of the areas under investigation were obtained after several cumulative demineralization times. Sixteen images were obtained from each dentin area of each tooth for each experimental time, at 1000x magnification. An image processing and analysis sequence measured sets of images, providing data of area fraction for thousands of tubules over time, allowing to quantitatively follow the effect of the chelating substances. The Kruskal-Wallis H-test and Dunn's multiple comparison test were used to analyze the data. Overall, it can be concluded that the demineralization kinetics promoted by both 9% HEBP and 18% HEBP were significantly slower than those of 17% EDTA (p < 0.05). In addition, the single-tooth model is advantageous over the first co-site optical microscopy dentin asse...

These results address the need for an improved quality assurance, in order for the students and faculty to improve their practices and attitudes on infection control measures.

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Objective: Earlier studies on some dental materials measured roughness and/or contact angles or fluoride release separately. In the present study, five dental polymers were investigated to ascertain their contact angles, wettability, roughness, and fluoride release in dry or wet conditions. Methods: Samples for 5 materials were prepared and stored dry or wet in deionized water pH 6.8. Samples were submitted to finishing/polishing procedures, and the measurements in Goniometer, roughness (µm) and fluoride analysis Results and conclusions: Except for the Ariston pHc, all the materials displayed high contact angles when measured with water, showing hydrophobic characteristics. Roughness changed the contact angles, especially those of Ariston (α < 0.05). Fluoride did not modify the contact angles, but increased the roughness of the finished material.