Microbial profile knowledge is essential to news alternatives and improvements in current treatments and destinations of landfill leachate that contains a variety of toxic compounds produced by municipal solid waste (MSW) disposal. Environmental DNA metabarcoding is an efficient, quick, and low-cost methodology for whole communities’ characterization. In this respect, the leachate from the Sanitary Landfill of Foz do Iguaçu City showed mixed characteristics from both acid and methanogenic phases, and 16S rDNA metabarcoding suggested the dominance of fermenting bacteria belonging to Firmicutes phylum, followed by Proteobacteria, Bacteroidetes and Synergistetes. The leachate acidogenic phase ended up being masked in the chemical and physical analyzes, however was evidenced in the metabarcoding methodology. On the other hand, no specifically methanogenic group was detected in significant abundance. To future application using culturomics approaches to bioremediation the leachate sample presented groups extensively studied, especially the Pseudomonas genus to heavy metals treatments, such as cadmium.

Microbial pro le knowledge is essential to news alternatives and improvements in current treatments and destinations of land ll leachate that contains a variety of toxic compounds produced by municipal solid waste (MSW) disposal. Environmental DNA metabarcoding is an e cient, quick, and low-cost methodology for whole communities' characterization. In this respect, the leachate from the Sanitary Land ll of Foz do Iguaçu City showed mixed characteristics from both acid and methanogenic phases, and 16S rDNA metabarcoding suggested the dominance of fermenting bacteria belonging to Firmicutes phylum, followed by Proteobacteria, Bacteroidetes and Synergistetes. The leachate acidogenic phase ended up being masked in the chemical and physical analyzes, however was evidenced in the metabarcoding methodology. On the other hand, no speci cally methanogenic group was detected in signi cant abundance. To future application using culturomics approaches to bioremediation the leachate sample presented groups extensively studied, especially the Pseudomonas genus to heavy metals treatments, such as cadmium.