Human hair dandruff (HHD) is a common unwanted scalp disorder that is prevalent to most human populations all over the world. This study was designed to isolate and characterize pathogens that are responsible for HHD as well as the evaluation of their biological control technique. Isolated bacteria were characterized by different biochemical tests and molecular identification methods. Here, disc diffusion methods were used to determine antibiotic and antibacterial activity against isolated bacteria. The isolated bacterial colonies were found to be Gram-positive, small, round-shaped, and purple. PCR amplification was done using 27F and 1492R primer pairs. A BlastN search of a sequenced 1465 bp region of 16S rRNA in NCBI GenBank revealed approximately 99% genome similarity with Staphylococcus aureus. The sequence was deposited in GenBank (Accession No. MH603394). In the antibiotic sensitivity test, Kanamycin showed the highest 31.0±0.5 mm diameter zone of inhibition (DZI) against the isolated bacteria. Moreover, as a plant-derived compound, the Methanol extract of Allium sativum revealed the highest, 15.0±0.5 mm DZI. The present study would give a promising direction of identification and control of this pathogen biologically.