This investigative laboratory exercise uses the different relations between ambient temperature and metabolic rate in endotherms and ectotherms as a core concept to answer the following question: What thermoregulatory mode is employed by chicken embryos? Emphasis is placed on the physiological concepts that can be taught with this exercise, including methods for measuring rates of oxygen consumption, the relation between oxygen consumption and metabolic rate, the influence of temperature on metabolic rate, and the differences between endotherms and ectotherms both in the overall magnitude of metabolic rate and in the shape of the relation between metabolic rate and ambient temperature. Included in this article are respirometer designs suitable for teachers working with a wide variety of budgets and available equipment, specific laboratory protocols for collecting data, sample data, thought questions with sample answers, and suggestions for classroom implementation as a 1-, 2- or 3-wk laboratory exercise that can be taught at a variety of undergraduate levels.
Answers to mechanistic questions about biological phenomena require fluency in a variety of molecular biology techniques and physical concepts. Here, we present an interdisciplinary approach to introducing undergraduate students to an important problem in the areas of animal behavior and neuroscience—the neuronal control of animal behavior. In this lab module, students explore planarian behavior by quantitative image and data analysis with freely available software and low-cost resources. Planarians are ∼1–2-cm-long aquatic free-living flatworms famous for their regeneration abilities. They are inexpensive and easy to maintain, handle, and perturb, and their fairly large size allows for image acquisition with a webcam, which makes this lab module accessible and scalable. Our lab module integrates basic physical concepts such as center of mass, velocity and speed, periodic signals, and time series analysis in the context of a biological system. The module is designed to attract students with diverse disciplinary backgrounds. It challenges the students to form hypotheses about behavior and equips them with a basic but broadly applicable toolkit to achieve this quantitatively. We give a detailed description of the necessary resources and show how to implement the module. We also provide suggestions for advanced exercises and possible extensions. Finally, we provide student feedback from a pilot implementation.
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