The objective of this study was to evaluate stigma receptivity and anther dehiscence in three floral development stages in F3 population of ornamental pepper (Capsicum annuum). A total of 162 plants (81 of purple corolla and 81 of white corolla) were used in this study. To analyze the stigma receptivity and anther dehiscence, three floral stages were analyzed: bud, pre-anthesis, and open flower. For each stage, 33 flowers of each phenotypic class were collected. The receptivity of the stigma was detected by dripping hydrogen peroxide solution (3%). For the evaluation of the level of receptivity, scores of 0 to 3 were assigned, varying according to the bubbles percentage found on the stigmatic surface. Scores of 1 to 5 were assigned for the reference of the amount of pollen released by the stamens. The data were subjected to analysis of variance (p≤0.05) following a 2x3 factorial scheme: two classes of flower colors and three development stages, with 33 replicates (flowers) with later separation of means by Tukey’s test at the same probability level. For the analysis of divergence, the data were clustered according to Tocher’s method based on generalized Mahalanobis distance. Despite the not significant differences between the means for pollen dehiscence of both flower colors, the purple flowers presented higher stigma receptivity than the white ones. The ornamental pepper flowers displayed stigma receptivity since the bud phase, but the highest level of receptivity was observed after anthesis. Anther dehiscence prevailed after anthesis of the flowers regardless of color, and the presence of pollen grains was superior after anthesis. For the manual crossing, the buds at pre-anthesis phase are the most indicated irrespective of the color, as they show high stigma receptivity and greater ease at handling.

<p>Este trabalho teve por objetivo avaliar o desenvolvimento de clones de <em>Pyrostegia venusta </em>em diferentes tipos de substratos. O experimento foi conduzido em delineamento inteiramente casualizado, com 06 tratamentos e 10 repetições, cada repetição foi constituída por um tubete de polietileno com capacidade de 103cm³. Os tratamentos testados foram: (T1) 100% de areia lavada; (T2) 50% areia lavada + 50% substrato comercial Plantmax®; (T3) 100% de substrato comercial Plantmax®; (T4) 50% areia lavada + 50% de esterco bovino; (T5) 1/3 areia lavada + 1/3 esterco bovino +1/3 de Plantmax® e (T6) 50% esterco bovino + 50% de Areia lavada. Após 30 dias foram avaliados o número de estacas sobreviventes, comprimento do broto (cm), número de folhas, comprimento da folha (cm), número de raízes e comprimento da raiz principal (cm).  Os dados foram submetidos a análise de variância e as médias comparadas pelo teste de Tukey a 5% de probabilidade. Por meio da análise de variância (p&lt;0,01), detectou-se significância estatística, para as variáveis, sobrevivência das estacas e número de brotos por estaca de <em>P. venusta</em>, quando cultivadas em substrato composto por 50% areia lavada + 50% substrato comercial Plantmax®. Essa mistura apresenta a vantagem de ser mais econômica quando comparado ao substrato comercial Plantmax®, sendo portanto, recomendada na produção de mudas de <em>cipó-de-São João</em><em>.</em></p><p align="center"><strong><em>Utilization of different substrates in the propagation of Pyrostegia venusta by cuttings</em></strong></p><p><strong>Abstract</strong><strong>: </strong>The experiment was carried out in a completely randomized design with 06 treatments and 10 replicates, each replicate consisted of polyethylene plastic tube the size of 103cm³. The treatments were: (T1) 100% washed sand; (T2) 50% washed sand + 50% commercial substrate Plantmax®; (T3) 100% substrate commercial Plantmax®; (T4) 50 % washed sand + 50% cattle manure; (T5) 1/3 washed sand + 1/3 manure +1/3 Plantmax® and Plantmax (T6) 50 % cattle manure + 50% washed sand. After 30 days, were evaluated number of cutting survival, bud length in cm, number of leaves, leaf length in cm, number of roots and length of the main root. Data were submitted to analysis of variance and means were compared by Tukey test at 5% probability. By analysis of variance (p&lt; 0.01), there was significant differences for the variables, czutting survival and number of shoots per cutting when cultured on substrate composed of 50% washed sand + 50% commercial substrate Plantmax®. Furthermore, this mixture has the advantage to be cheaper as compared to substrate Plantmax®, so we recommend the seedlings of Saint John’s vine.</p><p><em><br /></em></p>

Plant breeding of ornamental peppers (Capsicum spp.) can be supported by biotechnological tools. The objective of this study was to evaluate the efficiency of an in vitro culture of immature zygotic embryos (IZE) to reduce the breeding cycle of ornamental pepper (C. annuum) in comparison to the conventional system. Three ornamental pepper genotypes were used: UFPB 001, UFPB 004, and UFPB 099. Embryos at 30 days after selfing were inoculated in MS ½-strength culture medium, and at the same time, seeds were placed to germinate in a commercial substrate. Approximately 215 days are required from selfing until fruit ripening in the conventional system, whereas the IZE system requires an average of 153 days, a decrease of approximately 30% per selection cycle, corresponding to 496 days considering 8 selfing cycles. A decrease in time, labor, and inputs makes the IZE system a suitable tool for shortening the breeding program of ornamentals peppers.

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