The results indicate that electrogustometry and the filter paper disc method are reliable methods to measure taste with a high degree of reproducibility. The only actions causing significant changes in the electrogustometry readings were eating a bitter substance and having local anesthesia of the tongue. The correlation between the methods was statistically significant except for the bitter flavor, where the correlation was just below the level of significance.
The objective of this study was to examine factors affecting morbidity after tonsillectomy in children. Data from the National Tonsil Surgery Register in Sweden on 18,712 patients who underwent tonsillectomy with or without simultaneous adenoidectomy between 1 and 18 years of age were analysed. This register includes data on sex, gender, surgical indication, and the surgical and haemostasis techniques used for each patient, as well as patient-reported outcomes for haemorrhage, analgesic use and antibiotic use. Comparison of patients who underwent surgery for infection versus upper airway obstruction revealed a significant increase in haemorrhage complications in the infection group. However, no significant difference remained after the adjustments for confounders in multivariable regression analysis. Instead, the increased risk among patients who underwent surgery for infection was mainly attributable to the use of bipolar diathermy and increased patient age. Patients who received surgery for infection reported more days of analgesic use, as well as more unplanned contacts with a health care service provider due to pain, compared with those who underwent surgery for upper airway obstruction. These results remained significant in multivariate analysis. The use of bipolar diathermy for haemostasis resulted in an increased risk, while the use of cold steel surgical instruments, a younger patient age and female sex led to a decreased risk. The surgical and haemostasis techniques used are the most important factors that affect morbidity after tonsillectomy in the paediatric age group. The choice of surgical techniques is of utmost importance for decreasing morbidity in these patients.
Background: The outer epithelium of the tympanic membrane (TM) initiates the closure of a perforation. Embryonic stem cells have been used in attempts to enhance the healing capacity of induced perforations in experimental animals. More knowledge about epithelial cell proliferation and cell migration is needed for a better understanding of the TM healing process. This includes the identification of regenerative zones within the outer epithelial layer of the TM where progenitor cells may be present. Methods: Normal human TMs from translabyrinthine surgery were investigated using immunohistochemistry and immunofluorescence to detect the progenitor/stem cell markers α6-integrin, β1-integrin and cytokeratin 19 (CK19). Results: α6-Integrin was detected in the basal layer of the keratinizing epithelium in the umbo, in the annular region and along the malleus but not in the intermediate portion of the pars tensa. β1-Integrin and CK19 were found in the same locations not only in the basal layer but also in the suprabasal layers of the keratinizing epithelium. Conclusions: Possible progenitor cells are found in the umbo, the annular region and along the malleus. Further studies are needed to identify the source of these cells.
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