The onset of the COVID-19 pandemic drastically changed the circumstances for parents to combine work and care. We examined work-family guilt among working parents in the Netherlands during the first year of the COVID-19-pandemic (2020). In a cross-sectional survey study with two time points (March and December 2020) in a sample of working parents (N = 574) in the Netherlands, we revealed three key findings. (1) On average, mothers experienced more guilt towards their family for time and energy invested in work instead of family (work-family guilt) than fathers. (2) Parents with essential occupations (regardless of gender) reported significantly stronger experiences of work-family guilt during the first year of the pandemic compared to parents with non-essential occupations. (3) Parents who experienced stronger work-family guilt were more likely to compensate for their guilt by prioritizing family over work and self (by foregoing their leisure time or intending to reduce workhours in order to be with their family). Together these findings show that in terms of combining work and family, the situation is clearly harder on some more than others, namely mothers and essential workers. These findings demonstrate the gendered experience of work-family guilt and the asymmetrical impact of the COVID-19 pandemic on the work-family experiences of parents.