Dendritic cells (DC) are crucial gatekeepers in regulating immunity. Whereas mature immunostimulatory myeloid DC (DC ims ) potently promote immune responses, IL-10-induced myeloid DC (DC-IL-10) counteract T cell activation. To elucidate the molecular repertoire by which DC-IL-10 secure reduced T cell activation, comparative gene expression profiling was done using Affymetrix U133A microarrays. Among the genes overexpressed in DC-IL-10, eight immunoreceptor tyrosine-based inhibitory motif (ITIM)-containing inhibitory molecules (ILT2, ILT3, ILT4, ILT5, DCIR, PILRA, FccRIIB, SLAM) were found. Phenotypic analysis of DC-IL-10 defined an ILT high DC subset further characterized by expression of CD14, TLR2, DC-SIGN, and CD123 and the lack of lymphocyte, monocyte/macrophage, and plasmacytoid DC markers such as CD3, CD8, and CD68. A unique feature of the ILT high DC subset was expression of the novel DC marker BDCA3, which was not detected on monocytes, immature DC, DC ims , or ILT low DC-IL-10. While the allogeneic T cell proliferation induced by the entire DC-IL-10 population was approximately 30% of that stimulated by DC ims , allogeneic MLR responses driven by the ILT high DC subset were reduced to 8% of the allostimulatory capacity of DC ims , although secretion of the inhibitory cytokine IL-10 and other Th1/Th2-associated cytokines was similar in these cells.

No abstract