Background: AFN-1252 is a FabI inhibitor developed to treat Staphylococcus aureus. Results: AFN-1252 resistance arises from a single missense mutation that produces a FabI(M99T) protein. Conclusion:The interaction between Met-99 and AFN-1252 accounts for the staphylococcal selectivity of the drug. Significance: The AFN-1252-resistant strains remain susceptible to drug concentrations typically achieved in antibiotic therapy.

CONCLUSION:The intraocular pressure significantly increased during robotic surgery. Caution should be exercised during surgery on patients with glaucoma or increased intracranial pressure as baseline.