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The poly(ethylene glycol) (PEG)-modified enzyme superoxide dismutase (PEG-SOD) was characterized using capillary electrophoresis (CE), and the results were compared to those obtained using matrix-assisted laser desorption/ionization (MALDI) mass spectrometry. A free-solution CE method was developed which separated PEG-SOD species based on the number of attached PEG chains. The identity of individual CE peaks was established by isolation and subsequent molecular weight analysis by MALDI. The accuracy of the CE method in determining the average total number of PEG chains attached to the enzyme was established by comparing it with an HPLC method that quantitates the amount of PEG after hydrolytic cleavage from the modified enzyme. In addition to determining the average number of PEG chains attached to the protein, the CE method provides information on the distribution of PEG conjugation to the SOD. The total amount and distribution of coupling of PEG to the enzyme was also directly measured using MALDI. The distribution profile of PEG modification for a typical sample of PEG-SOD as determined by CE was found to be consistent with that obtained by MALDI. A quantitative comparison of the results obtained by the two techniques on PEG-SOD samples representing a range of values of PEG modification of the enzyme demonstrated good correlation, although differences were noted in some cases. Both techniques achieved a high degree of reproducibility when properly optimized. Data were also obtained on stressed samples of PEG-SOD demonstrating the ability of the CE method to detect changes in the degree and distribution of PEG conjugation for samples on stability. These two techniques provide a convenient means of characterizing the distribution of coupling of PEG to this enzyme which may have applications to other PEG modified proteins.Modification of protein-based pharmaceuticals by chemically conjugating methoxypoly(ethylene glycol) (m-PEG) chains to the protein has been demonstrated to be an effective procedure for reducing immunogenicity, increasing circulating half-life, and improving stability for these therapeutic agents. [1][2][3][4][5][6] The enzyme Cu, Zn-superoxide dismutase (SOD) catalyzes the destruction of superoxide anions which are byproducts of all oxygen-metabolizing organisms. Bovine SOD has been conjugated with m-PEG of average molecular weight 5000 via a linker to -amino groups of lysines to produce poly(ethylene glycol) superoxide dismutase (PEG-SOD). 7 [Superoxide dismutase from bovine origin as Cu-Zn mixed chelate with enzyme activity is also known as orgotein (United States Pharmacopeial Convention, Inc.; Fleeger, C. A., Ed.; The United States Pharmacopeial Convention: Rockville, MD, 1993; p 462). Hence, the PEG-conjugated bovine SOD is called pegorgotein. Pegorgotein is also identified as PEG-SOD in the literature. The Sanofi-Winthrop, Inc. proprietary name for PEG-SOD is Dismutec.] This agent has been evaluated as a free-radical scavenger to protect against the potential damaging effects of free radi...

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Abstract. A series of buffer systems encompassing the pH range from 3.5 to 9.0 have been developed for free solution capillary electrophoretic (CE) analysis of basic proteins in uncoated fused silica capillaries. Separations of model proteins possessing isoelectric points between 9.1-1 1 .O have been achieved with efficiencies in the range of 95,000-690,000 theoretical plates. In each case the modifier 1,3-diaminopropane is incorporated in the operating buffers at concentrations of 30-60 mM along with moderate levels of alkali metal salts to suppress protein-capillary wall interactions normally encountered with basic proteins. The combination of these buffer additives allows the analyses to be conducted at pHs below the protein isoelectric points. No special capillary pretreatment is required. The advantages of this approach to analyzing basic proteins include its simplicity, the ability to perform the analyses in the pH range where proteins are stable and the greater flexibility available in the choice of pH as a means of affecting selectivity changes.