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Front the Dcpartment of Prez;cittive Xedicine nnd Public Health, and the D e p a r t m n t of B ioclr c ni ist ry , 7'a 71 r7c r 71 i 1 t T -11 iz; r rs i t Sc h oo 1 of V c d icin c, Nnsh 2: i 1 I c , T e 11 11 .

The wide antibiotic spectrum of aureomycin prompted exploration for possible activity against B. tularense. Its evidently low toxicity and its absorption in active form when administered orally would be decided advantages should it prove to be effective in tularemia. EXPERIMENTAL STUDIESThe test organism. Studies were undertaken using a virulent strain of B. tularense recently recovered from a patient who died of tularemic meningitis (1). The organism was agglutinated by known tularemic serum to full titer. It failed to grow on blood agar but grew well on solid medium containing 8% fresh rabbit blood, 1% dextrose, and 0.1% 1-cystine in beef heart infusion agar base, prepared according to the method of Francis (2). Early cultures were preserved at -20°C. or 4°C. Fresh 24 to 48 hour transplants on blood dextrose cystine agar were used in all experiments, representing the second to seventh transfers from the original culture. The purity of each culture used was checked microscopically and on blood agar plates.

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