The Gesnerioideae includes most of the New World members of the Gesneriaceae family and is currently considered to include five tribes: Beslerieae, Episcieae, Gesnerieae, Gloxinieae, and Napeantheae. This study presents maximum parsimony and maximum likelihood phylogenetic analyses of nuclear ribosomal DNA internal transcribed spacer regions (ITS), and the chloroplast DNA trnL intron, trnL-trnF intergenic spacer region, and trnE-trnT intergenic spacer region sequences. The ITS and cpDNA data sets strongly support the monophyly of a Beslerieae/Napeantheae clade; an Episcieae clade; a Gesnerieae clade; a Gloxinieae clade minus Sinningia, Sinningia relatives, and Gloxinia sarmentiana; and a Sinningia/Paliavana/Vanhouttea clade. This is the first study to provide strong statistical support for these tribes/clades. These analyses suggest that Sinningia and relatives should be considered as a separate tribe. Additionally, generic relationships are explored, including the apparent polyphyly of Gloxinia. Chromosome number changes are minimized on the proposed phylogeny, with the exception of the n = 11 taxa of the Gloxinieae. Scaly rhizomes appear to have been derived once in the Gloxinieae sensu stricto. The number of derivations of the inferior ovary is unclear: either there was one derivation with a reversal to a superior ovary in the Episcieae, or there were multiple independent derivations of the inferior ovary.

Vitaceae (the grape family) consist of 16 genera and ca. 950 species primarily distributed in tropical regions. The family is well-known for the economic importance of grapes, and is also ecologically significant with many species as dominant climbers in tropical and temperate forests. Recent phylogenetic and phylogenomic analyses of sequence data from all three genomes have supported five major clades within Vitaceae: (i) the clade of Ampelopsis, Nekemias, Rhoicissus, and Clematicissus; (ii) the Cissus clade; (iii) the clade of Cayratia, Causonis, Cyphostemma, Pseudocayratia, Tetrastigma, and an undescribed genus "Afrocayratia"; (iv) the clade of Parthenocissus and Yua; and (v) the grape genus Vitis and its close tropical relatives Ampelocissus, Pterisanthes and Nothocissus, with Nothocissus and Pterisanthes nested within Ampelocissus. Based on the phylogenetic and morphological (mostly inflorescence, floral and seed characters) evidence, the new classification places the 950 species and 16 genera into five tribes: (i)

Tribe Gloxinieae has been estimated to include 22 genera and approximately 290 species. This study presents maximum parsimony phylogenetic analyses of nuclear ribosomal DNA internal transcribed spacer regions sequences, the chloroplast DNA trnL intron and trnL-trnF intergenic spacer region sequences, a morphological cladistic dataset, and combined analyses of these datasets. These analyses suggest that the genera Gloxinia, Phinaea, and (possibly) Diastema are polyphyletic; Kohleria is paraphyletic in relation to Capanea; Bellonia and Pheidonocarpa should be considered members of tribe Gesnerieae; and Lembocarpus is a member of tribe Episcieae. Furthermore, the historically recognized genus Seemannia, now included in Gloxinia, appears to form a strongly supported monophyletic group; several Gloxinia species from southern Brazil appear to be most closely related to Goyazia; Capanea, Kohleria, Pearcea s. l., and Diastema vexans appear to form a strongly supported clade; and Diastema, Monopyle, Phinaea (in part), and a few Gloxinia species (Gloxinia dodsonii, G. lindeniana, and G. racemosa) form a clade. Classification issues and generic boundaries of these lineages are discussed in detail.