Copper Chelates of dimethyl sulfoxide without compound. Sufficient buffer was used to give a total volume of 5.0 ml in each case.B. Ascites. The Ehrlich ascites tumor cells were maintained by weekly transfer of 0.1-0.2 ml of the cell-rich ascites fluid each from Swice white mice with 7-10-day tumors. For respiration studies, approximately 4 ml of the ascites fluid was withdrawn from one mouse following cervical dislocation. Normally, at least 500 X 106 cells (2-3 g wet) could be obtained. The cells were washed in isotonic saline before use and were suspended in 10 ml of saline and maintained in ice until used. From 0.2 to 0.4 ml of this suspension was sufficient for individual respiration rate determinations. In measurements of respiratory inhibition the cells were incubated with pure dimethyl sulfoxide (0.1-0.3 ml) as a control or with dimethyl sulfoxide plus chelate (0.1-0.3 ml) as treated in sufficient glucose-free phosphate-Ringer to give a total volume of 3.0 ml. As with the liver slice measurements, the incubation period was 30 min at 37°at which time the respiration rate was recorded using a Yellowsprings Model 53 oxygraph.

No abstract

This review is intended to focus attention on the importance of essential metallo-element metabolism, particularly copper metabolism, as an important component of normal bone metabolism in maintenance and repair. Literature published since Rademacher's early (1) astute observation that copper supplementation increases the rate of bone healing documents and explains key roles of copper-dependent processes required for maintenance and repair of this tissue. State of the art approaches to treatment of bone diseases including lengthening of bone and repair of fractures, can be improved by paying closer attention to the roles of copper and other essential metallo-elements required for optimal treatment.

A double-blind study was done giving 10 mg of copper/day as copper gluconate or placebo capsules for 12 wk. The seven subjects receiving copper gluconate had no change in the level of copper in the serum, urine, or hair. There was also no change in the levels of zinc or magnesium. There was also no significant change in levels of hematocrit, triglyceride, SGOT, GGT, LDH, cholesterol, or alkaline phosphatase. The side effects of nausea, diarrhea, and heartburn were the same in the subjects receiving copper gluconate and subjects receiving placebo capsules.

The review of over 800 references on aluminum (Al) published since the mid-fifties covers the occurrence of Al in soil, air, water, plants and food products, as well as air and water pollution problems. In addition, the existing quality criteria, the biology and toxicology of Al, and the therapeutic and medical uses are presented. It is concluded that absorption and retention or accumulation of Al in humans occurs at lower levels of intake than had been assumed formerly. However, levels of 5 to 50 times the normal daily intake do not appear to interfere with other metabolic processes. The adverse effects of Al reported in the more recent years resulted from the inhalation or ingestion of Al in concentrations many times greater than the amounts present under normal circumstances. As in the past, there is still no need for .ncern by the public or producers of Al or its products concerning hazards to human health derived from well established and extensively used products.