The Schizosaccharomyces pombe SMC proteins Rad18 (Smc6) and Spr18 (Smc5) exist in a high-M r complex which also contains the non-SMC proteins Nse1, Nse2, Nse3, and Rad62. The Smc5-6 complex, which is essential for viability, is required for several aspects of DNA metabolism, including recombinational repair and maintenance of the DNA damage checkpoint. We have characterized Nse2 and show here that it is a SUMO ligase. Smc6 (Rad18) and Nse3, but not Smc5 (Spr18) or Nse1, are sumoylated in vitro in an Nse2-dependent manner, and Nse2 is itself autosumoylated, predominantly on the C-terminal part of the protein. Mutations of C195 and H197 in the Nse2 RING-finger-like motif abolish Nse2-dependent sumoylation. nse2.SA mutant cells, in which nse2.C195S-H197A is integrated as the sole copy of nse2, are viable, whereas the deletion of nse2 is lethal. Smc6 (Rad18) is sumoylated in vivo: the sumoylation level is increased upon exposure to DNA damage and is drastically reduced in the nse2.SA strain. Since nse2.SA cells are sensitive to DNA-damaging agents and to exposure to hydroxyurea, this implicates the Nse2-dependent sumoylation activity in DNA damage responses but not in the essential function of the Smc5-6 complex.SUMO is a small ubiquitin-like protein that is covalently attached to target proteins. In yeasts and lower eukaryotes, SUMO is encoded by a single gene, while in higher eukaryotes there are three isoforms, SUMO-1, SUMO-2, and SUMO-3. The attachment of SUMO to target proteins is similar to the process of ubiquitination: SUMO is produced as a precursor protein which is processed to the mature form by SUMO proteases, revealing a diglycine motif. SUMO is subsequently activated by the formation of a thioester bond with a cysteine residue on the SUMO E1-like activator enzyme, a heterodimer known as SAE. SUMO is then passed to an E2-like SUMO conjugator, with which it also forms a thioester bond at a cysteine residue. SUMO ligases have been identified in several organisms. However, whereas E3 ligases are required for the attachment of ubiquitin to targets both in vitro and in vivo, the requirement for SUMO ligases for the attachment of SUMO to targets appears to be less stringent in vitro, and possibly also in vivo. This would be consistent with reports that several SUMO target proteins interact directly with the E2-like SUMO conjugator (e.g., see reference 4).Two classes of SUMO ligases have been identified. Proteins in the first category contain C3HC4-like RING domains, while proteins in the second category do not. Members of the first category include the Saccharomyces cerevisiae proteins Siz1 and Siz2 (16) and the mammalian PIAS family of proteins (20,32,38). Members of the second category include the RanBP2 and Pc proteins (18, 33). In S. cerevisiae (budding yeast), the SIZ1 and SIZ2 genes are not essential for viability, and null mutants do not show the severe cell and nuclear morphologies (16) that are observed with mutants that are defective in other components of the sumoylation system (17, 39). It remains...
The rad18 gene of Schizosaccharomyces pombe is an essential gene that is involved in several different DNA repair processes. Rad18 (Smc6) is a member of the structural maintenance of chromosomes (SMC) family and, together with its SMC partner Spr18 (Smc5), forms the core of a high-molecular-weight complex. We show here that both S. pombe and human Smc5 and -6 interact through their hinge domains and that four independent temperature-sensitive mutants of Rad18 (Smc6) are all mutated at the same glycine residue in the hinge region. This mutation abolishes the interactions between the hinge regions of Rad18 (Smc6) and Spr18 (Smc5), as does mutation of a conserved glycine in the hinge region of Spr18 (Smc5). We purified the Smc5-6 complex from S. pombe and identified four non-SMC components, Nse1, Nse2, Nse3, and Rad62. Nse3 is a novel protein which is related to the mammalian MAGE protein family, many members of which are specifically expressed in cancer tissue. In initial steps to understand the architecture of the complex, we identified two subcomplexes containing Rad18-Spr18-Nse2 and Nse1-Nse3-Rad62. The subcomplexes are probably bridged by a weaker interaction between Nse2 and Nse3.
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