Abstract.  1. Pollen can be transported thousands of kilometres by insects but its viability after long‐distance transport is not known. Knowing the potential for this mechanism to cause outcrossing of transgenes from genetically modified (GM) plants is important for risk assessments.2. The viability of pollen from cotton (Gossypium hirsutum L.) and canola (Brassica napus L.) was determined after placing it on the proboscis of Helicoverpa armigera moths for intervals of up to 32 h. Viability of both cotton and canola pollen declined at a much greater rate when in contact with the moth proboscis. Most was non‐viable by 8 h compared with 16 h for control cotton pollen or 32 h for canola pollen.3. There was no significant difference in the rate of decline of pollen viability between the five conventional cotton varieties, or between these and the one GM cotton variety used in these experiments.4. The number of canola pollen grains remaining on the proboscis declined over time. Very few cotton pollen grains were retained on the proboscis.5. The reduction in pollen viability during contact with the proboscis might indicate partial ingestion of the pollen via the proboscis.6. The points above suggest that pollen is unlikely to remain attached or remain viable when carried over large distances by H. armigera. The implications for spread of pollen from transgenic plants and for pollination ecology in general are discussed.

No abstract

Site-specific measurements of the apparent electrical conductivity (ECa) of soil using the EM38 were correlated with near-simultaneous neutron probe readings over periods of moisture extraction by an irrigated cotton crop. Thirty sites were monitored from three ECa zones within a 96-ha field of grey Vertosol soil 30 km west of Moree, New South Wales, Australia. This study differs from previous approaches by reporting the effect on ECa of a wetting front (irrigation) reaching a single ECa measurement point in a field and by using polyethylene neutron probe access tubes so that the EM38 could be operated directly over the same site measured by a neutron probe. We report strong correlations (r = 0.94) between neutron probe counts (CRR) averaged to a depth of 40 or 60 cm and ECa from an EM38 held in the vertical mode 20 cm above the soil surface. All combinations of EM sensor height (0–1.2 m) to neutron probe measurement depth (0.2–1.4 m) returned correlations >0.85. The relationship between CCR and ECa was linear for the purposes of estimating water content over a range of background ECa levels. More critical modelling suggested a slight curve (logarithmic model) fitted best. The range of surface-surveyed ECa from the start of irrigation (refill point) to fully irrigated (full point) was ~27 mS m–1 for this Vertosol, where surface ECa readings typically ranged from 50 to 200 mS m–1. We suggest that the calibration of ECa to CRR might be effected by a two-point measurement of the soil, namely at both upper (field capacity) and lower (wilting point) ECa values, and a site-specific calibration template generated by extending these point measures to whole-field surveys.

This paper is the third in a series designed to demonstrate the application of rigorous, systematic hazard identification techniques to ecological systems. Here we use Hierarchical Holographic Modelling to identify the potential ecological hazards associated with the commercial release of herbicide tolerant oilseed rape. Hierarchical Holographic Models decompose complex systems into a series of sub-systems and consider interactions between the components and processes of these sub-systems in order to identify hazards. In this example we considered 1356 potential interactions between the biological, chemical and physical components and processes of the herbicide tolerant oilseed rape environment, and identified 152 potential hazards, grouped into 14 categories. The hazards were subsequently scored for degree of concern and plausibility, and then compared with an equivalent list of hazards generated independently by a checklist approach. The incidence of herbicide tolerant volunteers (and weeds) both on and off the farm had the highest average score of all the ecological hazard categories. The checklist based approach identified or implied 44% of the hazards identified in the Hierarchical Holographic Model, including nine of the ten hazards ranked most important. The checklist approach focussed almost exclusively on the phenotypic and genotypic hazards associated with herbicide tolerant oilseed rape and largely ignored the hazards associated with the circumstances surrounding its use. As a result the checklist identified only 6 out of the 79 potential hazards associated with changes to farming practice. The commercial release of herbicide tolerant oilseed rape will be associated with changes in tillage and the application of post-emergent herbicides. It may also lead to changes in spray schedules of insecticide and fungicide. Many of the environmental hazards identified with these changes are plausible and may warrant further investigation or targeted monitoring.