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Low-speed sedimentation equilibrium experiments have yielded apparent weight-average molecular weight data (Mw;cia) at 5, 10,15, 25, and 35.5°as a function of concentration for 3-lactoglobulin B in pH 2.64 NaCl-glycine buffer, ionic strength 0.16. The concentration dependence of Mw(c)a is ascribed to self-association under nonideal conditions. The data are analyzed by using three different theoretical ap-proaches, with emphasis being given to the monomer-dimer scheme. The magnitude of the association constant » is sufficiently large that the results from the different formulations are subject to appreciable variation, depending upon the mathematical expression and the concentration range involved. These variations are discussed, and the results are compared to those of previous workers.2634 BIOCHEMISTRY, VOL.

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