Thirteen field trials were conducted in 1999 and 2000 to evaluate postemergence (POST) weed control with single applications of bromoxynil at 420 or 560 g ai/ha, glufosinate at 291 or 409 g ai/ha, glyphosate at 1,120 g ai/ha, pyrithiobac at 36 or 72 g ai/ha, or sulfosate at 1,120 g ai/ha. Additional treatments evaluated included two applications with glufosinate at both rates in all possible combinations, two applications of glyphosate, and two applications of sulfosate. Weeds were 2 to 5 cm or 8 to 10 cm tall for annual grass and broadleaf weeds whereas yellow nutsedge and glyphosate-resistant corn were 8 to 10 cm tall. All herbicide treatments controlled 2- to 5-cm common cocklebur, Florida beggarweed, jimsonweed, ladysthumb smartweed, Pennsylvania smartweed, pitted morningglory, prickly sida, redroot pigweed, smooth pigweed, and velvetleaf at least 90%. All herbicide treatments except pyrithiobac at either rate controlled 2- to 5-cm common lambsquarters, common ragweed, and tall morningglory at least 90%; pyrithiobac at the lower rate was the only treatment that failed to control entireleaf and ivyleaf morningglory at least 90%. Bromoxynil and pyrithiobac at either rate controlled 2- to 5-cm sicklepod 33 to 68% whereas glufosinate, glyphosate, and sulfostate controlled ≥99%. Glyphosate and sulfosate applied once or twice controlled hemp sesbania less than 70% and volunteer peanut less than 80%. Bromoxynil and pyrithiobac were the least effective treatments for control of annual grass species and bromoxynil controlled Palmer amaranth less than 80%. Glufosinate controlled broadleaf signalgrass, fall panicum, giant foxtail, green foxtail, large crabgrass, yellow foxtail, seedling johnsongrass, Texas panicum, and glyphosate-resistant corn at least 90% but controlled goosegrass less than 60%. Glyphosate and sulfosate controlled all grass species except glyphosate-resistant corn at least 90%. In greenhouse research, goosegrass could be controlled with glufosinate POST plus a late POST-directed treatment of prometryn plus monosodium salt of methylarsonic acid.

Measurement of shikimic acid accumulation in response to glyphosate inhibition of 5-enolpyruvylshikimate-3-phosphate synthase is a rapid and accurate assay to quantify glyphosate-induced damage in sensitive plants. Two methods of assaying shikimic acid, a spectrophotometric and a high-performance liquid chromatography (HPLC) method, were compared for their accuracy of recovering known amounts of shikimic acid spiked into plant samples. The HPLC method recovered essentially 100% of shikimic acid as compared with only 73% using the spectrophotometric method. Relative sensitivity to glyphosate was measured in glyphosate-resistant (GR) and non-GR cotton leaves, fruiting branches, and squares (floral buds) by assaying shikimic acid. Accumulation of shikimic acid was not observed in any tissue, either GR or non-GR, at rates of 5 mM glyphosate or less applied to leaves. All tissues of non-GR plants accumulated shikimic acid in response to glyphosate treatment; however, only fruiting branches and squares of GR plants accumulated a slight amount of shikimic acid. In non-GR cotton, fruiting branches and squares accumulated 18 and 11 times, respectively, more shikimic acid per micromolar of translocated glyphosate than leaf tissue, suggesting increased sensitivity to glyphosate of reproductive tissue over vegetative tissue. GR cotton leaves treated with 80 mM of glyphosate accumulated 57 times less shikimic acid per micromolar of translocated glyphosate than non-GR cotton but only 12.4- and 4-fold less in fruiting branches and squares, respectively. The increased sensitivity of reproductive structures to glyphosate inhibition may be due to a higher demand for shikimate pathway products and may provide an explanation for reports of fruit abortion from glyphosate-treated GR cotton.

An experiment conducted at five locations in North Carolina during 1998 and 1999 evaluated weed management systems in cotton with CGA-362622 and pyrithiobac. Weed management systems evaluated different combinations with or without fluometuron preemergence (PRE) followed by (fb) CGA-362622 early postemergence (EPOST), postemergence (POST), or EPOST + POST; or pyrithiobac EPOST fb prometryn plus MSMA late postemergence directed (LAYBY) or no LAYBY treatment. The weed species evaluated include common ragweed, entireleaf morningglory, pitted morningglory, prickly sida, sicklepod, tall morningglory, and yellow nutsedge. Fluometuron PRE improved the control of all weed species by at least 17 percentage points and increased cotton lint yield compared with the systems that did not use fluometuron PRE. Prometryn plus MSMA LAYBY improved the control of all weed species and increased lint yield compared with the systems that did not use prometryn plus MSMA LAYBY when PRE or POST herbicides were used. Control with CGA-362622 at all application timings was greater than 70% for all weed species evaluated (common ragweed, entireleaf morningglory, pitted morningglory, sicklepod, tall morningglory, and yellow nutsedge), except prickly sida. Control of all three morningglory species and prickly sida was at least 70% with pyrithiobac, whereas control of common ragweed, sicklepod, and yellow nutsedge was lower. The only cotton that yielded over 800 kg ha−1 was treated with fluometuron PRE fb CGA-362622 EPOST, POST, or EPOST + POST fb prometryn plus MSMA LAYBY. Cotton treated with pyrithiobac EPOST gave yields that were similar to those given by cotton treated with CGA-362622 EPOST in systems with fluometuron PRE and less than those given by cotton treated with CGA-362622 EPOST in systems without fluometuron PRE. Early-season injury with CGA-362622 was greater than 60% at Clayton and Rocky Mount in 1998, whereas 12% or less injury was observed at the other locations. Pyrithiobac resulted in 25 to 45% injury at these two locations. No injury was observed 45 d after treatment.