This paper is part of a program of research designed to develop a self-report measure of individual differences in motivational reactivity. Theoretically, individual differences in appetitive and aversive activation have been linked to personality types (e.g., sensation seeking) and to various types of behavior (e.g., risky behavior, substance use, and abuse). The difficulty in measuring individual differences in motivational activation (usually assessed by measuring neurochemical levels) has made it difficult to test hypotheses that posit variations in Correspondence should be addressed to Annie Lang, some process (such as media use or media viewing) as a function of differences in motivational activation. This study reports an investigation into the construct validity of MAM the Motivational Activation Measure. MAM is measured by using participants' self-reported ratings of emotional experience in response to 90 emotional pictures to calculate two measures: positivity offset, an estimate of activation in the appetitive system, and negativity bias, an estimate of the speed of activation of the aversive system. Results show that the negativity bias measure correctly predicts greater activation in physiological measures associated with aversive activation (e.g., startle potentiation, corrugator activity, heart rate acceleration) while the positivity offset measure correctly predicts physiological and behavioral indicators of appetitive activation (e.g., inhibition of startle, zygomatic activation, greater heart rate decelerations, and faster STRTs). Recent theories of motivated cognition argue that individual differences in the activation and reactivity of the appetitive and aversive motivational systems modulate how individuals perceive, attend to, encode, store, and respond emotionally to mediated messages (Lang, 2005a & b;Lang, Simons, & Balaban, 1997). It's old news that negative media messages can elicit avoidance behavior in people while positive messages elicit approach behavior and that there is a great deal of individual variation in the strength of these responses. This variation is evident at the behavioral level where we have all seen people who go to great lengths to avoid negative stimuli while others seem to seek them out. What's new is the claim that this individual-level variation in motivational activation affects cognitive and emotional processing long before any behavioral outcome is observed.We already have good evidence that individual differences in motivational activation and reactivity are related to risky behavior, substance abuse, and emotional experience, but little is known about how or even if individual differences in motivational activation affect the processing of mediated messages. Given the ubiquity of emotion in media messages of all kinds (from the news, to advertising, to health communication, to edutainment, and, of course, entertainment) it behooves us to learn more about individual differences in how mediated emotional content alters cognitive processing and emotional respo...