Spodoptera frugiperda (J.E. Smith, 1797) (Lepidoptera: Noctuidae) is a pest of great economic importance which can feed on more than 300 plant species. As it is polyphagous, its host plants may have variable physical and chemical constitutions. This may influence larval development, as protein and carbohydrate levels are important factors for adequate biological development. The aim of this study was to evaluate insect developmental parameters as well as to compare the food consumption of S. frugiperda larvae reared using diets with different protein levels under laboratory conditions. Three artificial diet formulations were used: one typically used for routine laboratory rearing, based on bean, wheat germ and brewer’s yeast (D1); one containing half the original amount of protein (D2), and the other with twice the original amount of protein (D3). The relative consumption rate (RCR), relative growth rate (RGR), and efficiency of conversion of ingested food (ECI) for S. frugiperda fourth instar larvae varied among diets. The protein present in the diet influenced the duration of larval and pupal periods and pupal weight, but did not affect larval survival, fecundity and longevity of adults. The different protein levels in the diets did not negatively influence population growth, so these three diet variations can be used for mass rearing in the laboratory. However, the influence of these diets on successive generations of the insect remains untested.
Chemical and biological insecticides have been frequently used in sugarcane fields to control insects-pests, including the sugarcane borer, Diatraea saccharalis. Among the products used, those based on chlorantraniliprole and Metarhizium anisopliae entomopathogenic fungus, stand out. Euborellia annulipes is an insect of the order Dermaptera considered a potential predator of sugarcane borer eggs. This study aimed to evaluate the direct and indirect effects of the bioinsecticide based on M. anisopliae (Metarril®) and the chemical insecticide chlorantraniliprole (Altacor®) on the mortality of E. annulipes nymphs and adults, the predation and feeding preference of earwigs in eggs treated with the formulated products. Predator mortality was evaluated for seven days after treatment, while the effect on predation was analyzed by preference tests with and without choice, using prey eggs. The products tested were selective to the predator, causing ≤ 2% mortality and not affecting predation. The application of M. anisopliae on sugarcane borer eggs favored the food preference of fourth-instar nymphs, males, and females of the predator.Our results show that Metarril® and Altacor® can be used to control D. saccharalis when associated with the predator E. annulipes.
Cladomorphus phyllinus Gray, 1835 (Phasmatodea: Phasmatidae) is a species of stick bug native to Brazil and considered one of the largest in the southern hemisphere. The rearing method described uses rectangular cages for the maintenance of nymph and adult insects, with feeding carried out with guava leaves. This methodology promotes the multiplication of insects, which have been used for educational purposes.
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