We present an all-polymer optical fiber sensor for the sensing of dissolved oxygen by phase-fluorometry. The sensing matrix is applied as a film on the fiber end-surface, and consists of poly-methylmethacrylate (PMMA), the oxygen quenchable luminophore platinum-octaethylporphyrin (PtOEP) and the luminophore coumarin 545T for increasing the brightness of PtOEP by way of resonance energy transfer (RET), also called light harvesting. We show that by using Hansen Solubility Parameters (HSPs), it is possible to quantitatively formulate a solvent mixture with a good solubility of the polymer matrix and the luminophores simultaneously. Our approach can readily be extended to other polymers and luminophores and is therefore a valuable tool for researchers working with photoluminescence and polymeric matrices.
Our aim is an efficient online cortisol sensor for aquaculture. We present a polymer optical fiber sensor design for cortisol using a competitive assay consisting of fluorophore tagged cortisol and a cortisol aptamer.
We present an all-polymer optical fiber sensor for the measurement of dissolved oxygen. The sensing matrix containing oxygen-sensitive PtOEP, is of the same polymer as the optical fiber core, leading to increased robustness.
In this study, we present a polymer optical fiber fluorophore/aptamer competitive assay-based cortisol sensing principle. We developed a low-cost, two-fiber perpendicular design for fluorophore-based sensing with less input light interference and high output signal intensity. The design is suitable for narrow stokes shift fluorophores. We have demonstrated the cortisol sensing principle based on the competition between tagged and normal cortisol. To date, the sensing design has exhibited a slow response, and we identified possible modifications for improvement. Our estimation shows that with miniaturization and a modified sensor assay compartment design, a less than one-hour response time can be achieved. The reported sensing principle and low-cost new design will be helpful for the future development of fluorophore-based fiber optic aptasensors that can potentially be used in a wet environment for online sensing.
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